生物技术进展
生物技術進展
생물기술진전
2014年
5期
318-324
,共7页
油体%油体蛋白%油体表达系统%人工油体%乳化剂
油體%油體蛋白%油體錶達繫統%人工油體%乳化劑
유체%유체단백%유체표체계통%인공유체%유화제
oil bodies%oleosins%oil bodies expression system%artificial oil bodies%emulsifying agents
油体是植物种子尤其是油料植物种子的重要贮脂细胞器,具有较强的物化稳定性,而且易于通过离心法分离提取。研究表明,油体是由外层的磷脂和油体结合蛋白以及包裹在内部的液态基质(主要为三酰甘油)形成的弹性球体或椭球体。目前,在植物中共发现三类油体结合蛋白,它们主要存在于油体表面。鉴于油体和油体结合蛋白的结构特殊性,二者在生物技术领域得到了广泛应用。本文重点综述了油体在表达纯化外源蛋白方面的优势、策略以及在生产药用蛋白、制备固定化酶、捕捉抗体和药用酶、生产营养素和提高植物抗性等多个领域的研究进展,并介绍了人工油体和油体乳化剂方面的开发应用情况。
油體是植物種子尤其是油料植物種子的重要貯脂細胞器,具有較彊的物化穩定性,而且易于通過離心法分離提取。研究錶明,油體是由外層的燐脂和油體結閤蛋白以及包裹在內部的液態基質(主要為三酰甘油)形成的彈性毬體或橢毬體。目前,在植物中共髮現三類油體結閤蛋白,它們主要存在于油體錶麵。鑒于油體和油體結閤蛋白的結構特殊性,二者在生物技術領域得到瞭廣汎應用。本文重點綜述瞭油體在錶達純化外源蛋白方麵的優勢、策略以及在生產藥用蛋白、製備固定化酶、捕捉抗體和藥用酶、生產營養素和提高植物抗性等多箇領域的研究進展,併介紹瞭人工油體和油體乳化劑方麵的開髮應用情況。
유체시식물충자우기시유료식물충자적중요저지세포기,구유교강적물화은정성,이차역우통과리심법분리제취。연구표명,유체시유외층적린지화유체결합단백이급포과재내부적액태기질(주요위삼선감유)형성적탄성구체혹타구체。목전,재식물중공발현삼류유체결합단백,타문주요존재우유체표면。감우유체화유체결합단백적결구특수성,이자재생물기술영역득도료엄범응용。본문중점종술료유체재표체순화외원단백방면적우세、책략이급재생산약용단백、제비고정화매、포착항체화약용매、생산영양소화제고식물항성등다개영역적연구진전,병개소료인공유체화유체유화제방면적개발응용정황。
Oil bodies are the important oil storage organelles in plant seeds, particularly in oil plant seeds. Oil bodies have strong physical and chemical stability and are easily separated by centrifugation. Studies showed that oil bodies have a spherical or ellipsoid shape and are composed of outer phospholipid, integral proteins and inner liquid matrix (mostly triacylglycerol). To date,three types of integral proteins have been found in plants and are mainly localized on the surface of oil bodies. In view of the unique structures of oil bodies and integral proteins,both of them have been widely applied in the field of biotechnology. This review summarized advantages and strategies of using oil bodies in expression and purification of recombinant proteins,as well as its applications in the fields of production of pharmaceutical protein, preparation of immobilized enzymes, affinity capture of antibodies and pharmaceutical enzymes, production of nutraceuticals, enhancement of plant resistance against pathogen. The development and applications of artificial oil bodies and oil-body-emulsifying-agents were also discussed.