CAJ | 학술논문

目的：建立同时测定蒙药嘎日迪五味丸中莽草酸和没食子酸的HPLC方法，为该蒙药复方制剂的质量控制提供一种简便、快速、准确的方法.方法：采用岛津LC-10ATVP高效液相色谱仪，色谱柱为ODS2（250mm×4.6mm，5μm）（大连中汇达科学仪器公司）；SPD-10AVP检测器；柱温25℃.结果：莽草酸在0.1～0.5μg，没食子酸在0.05～0.25μg范围内，莽草酸和没食子酸的浓度与相应的峰面积呈良好的线性关系.莽草酸的回归方程为Y=1.0×107 X-2.1×104, r=0.9999；没食子酸的回归方程为Y=9.0×108 X+3.8×105，r=0.9997，莽草酸、没食子酸的平均回收率分别为101.0%,RSD=1.60%；101.6%，RSD=1.75%.结论：本法简便、快捷、重复性好，结果准确可靠，可为研究蒙药嘎日迪五味丸的质量标准方法的建立提供可靠依据.
목적：건립동시측정몽약알일적오미환중망초산화몰식자산적HPLC방법，위해몽약복방제제적질량공제제공일충간편、쾌속、준학적방법.방법：채용도진LC-10ATVP고효액상색보의，색보주위ODS2（250mm×4.6mm，5μm）（대련중회체과학의기공사）；SPD-10AVP검측기；주온25℃.결과：망초산재0.1～0.5μg，몰식자산재0.05～0.25μg범위내，망초산화몰식자산적농도여상응적봉면적정량호적선성관계.망초산적회귀방정위Y=1.0×107 X-2.1×104, r=0.9999；몰식자산적회귀방정위Y=9.0×108 X+3.8×105，r=0.9997，망초산、몰식자산적평균회수솔분별위101.0%,RSD=1.60%；101.6%，RSD=1.75%.결론：본법간편、쾌첩、중복성호，결과준학가고，가위연구몽약알일적오미환적질량표준방법적건립제공가고의거.
Objective:To establish the simultaneous determination method of shikimic acid and gallic acid in Garidi-5 pills by HPLC, and to provide a simple, rapid and accurate method for the quality control of Mongolian compound prep-arations. Methods: Shimadzu LC-10ATVP HPLC was used, the column was ODS2 (250mm × 4.6mm,5μm, Dalian Delta Scientific Instrument Company);detector was SPD-10AVP;column temperature was 25℃. Results:the gshikim-ic acid in the range of 0.1-0.5μg, the gallic acid in the range of 0.05-0.25μg, the concentration and the correspond-ing peak area showed a good linear relationship, the regression equation of shikimic acid was Y=1.0 × 107 X-2.1 × 104,r=0.9999;the regression equation of gallic acid was Y=9.0×108 X+3.8×105, r=0.9997;The average recovery of shikimic acid and gallic acid were 101.0%(RSD=1.60%)and 101.6%(RSD=1.75%.) respectively. Conclusion:The method is easy, rapid and reproducible, the result is accurate and reliable, which can provide a good basis for establish-ing the quality standard control for Garidi-5 pills.