中国有色金属学报（英文版）
中國有色金屬學報（英文版）
중국유색금속학보（영문판）
TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
2014年
10期
3332-3342
,共11页
酸性矿业废水%微生物结构组成%克隆文库%理化因子
痠性礦業廢水%微生物結構組成%剋隆文庫%理化因子
산성광업폐수%미생물결구조성%극륭문고%이화인자
acid mine drainage%microbial community%clone library%geochemical variables
为了加深对安徽省铜陵市不同重金属富集程度的尾矿附近酸性废水中微生物多样性的了解，运用PCR-16S rDNA克隆文库技术对酸性废水中细菌及古菌的群落结构进行研究。在铜陵的5个样点采集了8个水样。系统发育分析结果表明，酸性废水中的细菌主要是Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi，古菌是Thermoplasma, Ferroplasma 以及Thermogymnomonas。根据细菌和古菌的组成对样品进行聚类分析，结果表明，5个样品由于优势菌株 Thermomonas 和 Meiothermus 拥有较高的相似度。2个样品由于 Acidithiobacillus 和Leptospirillum 占主导也拥有较高的相似度。剩余的样品多样性较高，拥有最高的香浓-微纳指数2.91。典型相关分析CCA的结果表明微生物的多样性组成与理化因子有密切的关系，如水的pH以及Hg2+、Pb2+、Cl-、SO42-、Fe3+的含量。
為瞭加深對安徽省銅陵市不同重金屬富集程度的尾礦附近痠性廢水中微生物多樣性的瞭解，運用PCR-16S rDNA剋隆文庫技術對痠性廢水中細菌及古菌的群落結構進行研究。在銅陵的5箇樣點採集瞭8箇水樣。繫統髮育分析結果錶明，痠性廢水中的細菌主要是Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi，古菌是Thermoplasma, Ferroplasma 以及Thermogymnomonas。根據細菌和古菌的組成對樣品進行聚類分析，結果錶明，5箇樣品由于優勢菌株 Thermomonas 和 Meiothermus 擁有較高的相似度。2箇樣品由于 Acidithiobacillus 和Leptospirillum 佔主導也擁有較高的相似度。剩餘的樣品多樣性較高，擁有最高的香濃-微納指數2.91。典型相關分析CCA的結果錶明微生物的多樣性組成與理化因子有密切的關繫，如水的pH以及Hg2+、Pb2+、Cl-、SO42-、Fe3+的含量。
위료가심대안휘성동릉시불동중금속부집정도적미광부근산성폐수중미생물다양성적료해，운용PCR-16S rDNA극륭문고기술대산성폐수중세균급고균적군락결구진행연구。재동릉적5개양점채집료8개수양。계통발육분석결과표명，산성폐수중적세균주요시Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi，고균시Thermoplasma, Ferroplasma 이급Thermogymnomonas。근거세균화고균적조성대양품진행취류분석，결과표명，5개양품유우우세균주 Thermomonas 화 Meiothermus 옹유교고적상사도。2개양품유우 Acidithiobacillus 화Leptospirillum 점주도야옹유교고적상사도。잉여적양품다양성교고，옹유최고적향농-미납지수2.91。전형상관분석CCA적결과표명미생물적다양성조성여이화인자유밀절적관계，여수적pH이급Hg2+、Pb2+、Cl-、SO42-、Fe3+적함량。
To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach. Eight acid mine water samples were collected from five areas in Tongling. Phylogenetic analyses revealed that bacteria mainly fell into ten divisions, which wereBetaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi.Archaea fell into three phylogenetic divisions,Thermoplasma, FerroplasmaandThermogymnomonas. The unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on the microbial communities’ compositions revealed that five samples shared similarity with the dominance ofMeiothermus and Thermomonas. Two samples had the preponderant existence ofAcidithiobacillus andLeptospirillum. The remaining sample owned higher microbial communities’ diversity with the Shannon-WeaverH up to 2.91. Canonical correlation analysis (CCA) suggested that microbial community structures had great association with pH and the concentration of Hg2+, Pb2+, Fe3+, Cl-, SO42-in water.
