CAJ | 학술논문

目的：建立臭蒿中东莨菪内酯成分的HPLC定性定量分析方法。方法：色谱柱为Agilent TC-C18柱（4.6 mm×250 mm，5μm），柱温30℃，流动相为甲醇（A）-0.3%磷酸溶液（B）梯度洗脱，流速1.0 mL·min-1，检测波长344 nm。结果：东莨菪内酯质量浓度与峰面积在测定范围内呈良好的线性关系（r=0.9999），重现性好，RSD值为1.51%；平均加样回收率为101.42%。结论：本试验建立的HPLC方法稳定可靠、简便易行，为臭蒿药材的质量评价和质量控制提供了理论依据。
목적：건립취호중동랑탕내지성분적HPLC정성정량분석방법。방법：색보주위Agilent TC-C18주（4.6 mm×250 mm，5μm），주온30℃，류동상위갑순（A）-0.3%린산용액（B）제도세탈，류속1.0 mL·min-1，검측파장344 nm。결과：동랑탕내지질량농도여봉면적재측정범위내정량호적선성관계（r=0.9999），중현성호，RSD치위1.51%；평균가양회수솔위101.42%。결론：본시험건립적HPLC방법은정가고、간편역행，위취호약재적질량평개화질량공제제공료이론의거。
In this paper, a qualitative and quantitative analysis method was established for the scopoletin in herba Artemisiae hedinii hy HPLC. The chromatographic column was Agilent TC-C18 (4.6 mm×250 mm, 5μm);the tem-perature of column was 30℃, eluted with a mobile phase consisted of methanol and 0.3%phosphoric acid solution and gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was 344 nm. The results showed that the mass concentration and peak area of scopoletin had a good linear relationship within the range of measurement (r=0.999 9). The reproducibility was good;and the RSD was 1.51%. The average sample recovery rate was 101.42%. It was concluded that the established method was stable, reliable and simple. It provided theoretical evidences for the quality evaluation and quality control of herba A. hedinii.