世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
10期
2223-2226
,共4页
双夏汤%葫芦巴碱%尿嘧啶%提取率%配伍
雙夏湯%葫蘆巴堿%尿嘧啶%提取率%配伍
쌍하탕%호호파감%뇨밀정%제취솔%배오
Shuangxia Soup%trigonelline%uracil%extraction yield%compatibility
目的：研究双夏汤中不同配比的半夏配伍夏枯草对葫芦巴碱、尿嘧啶提取率的影响，探索其变化规律，指导双夏汤的合理配伍。方法：采用Welch Ultimate AQ-C18色谱柱（4.6 mm×250 mm，5μm），以乙腈-水为流动相，梯度洗脱，流速为1.0 mL·min-1，检测波长为265 nm，柱温25℃，利用建立的HPLC法测定葫芦巴碱、尿嘧啶的提取率，比较得出不同配比对2种成分提取率的影响。结果：葫芦巴碱进样量在0.872-4.36 ng，尿嘧啶在10.8-50.4 ng之间线性关系良好，平均加样回收率分别为96.53%-101.48%， RSD均在0.18%-4.26%。双夏汤配伍后较半夏原药葫芦巴碱提取率降低，尿嘧啶提取率增加。结论：复方双夏汤不同配伍对葫芦巴碱和尿嘧啶的提取率有一定影响，最佳配比值得深入研究。
目的：研究雙夏湯中不同配比的半夏配伍夏枯草對葫蘆巴堿、尿嘧啶提取率的影響，探索其變化規律，指導雙夏湯的閤理配伍。方法：採用Welch Ultimate AQ-C18色譜柱（4.6 mm×250 mm，5μm），以乙腈-水為流動相，梯度洗脫，流速為1.0 mL·min-1，檢測波長為265 nm，柱溫25℃，利用建立的HPLC法測定葫蘆巴堿、尿嘧啶的提取率，比較得齣不同配比對2種成分提取率的影響。結果：葫蘆巴堿進樣量在0.872-4.36 ng，尿嘧啶在10.8-50.4 ng之間線性關繫良好，平均加樣迴收率分彆為96.53%-101.48%， RSD均在0.18%-4.26%。雙夏湯配伍後較半夏原藥葫蘆巴堿提取率降低，尿嘧啶提取率增加。結論：複方雙夏湯不同配伍對葫蘆巴堿和尿嘧啶的提取率有一定影響，最佳配比值得深入研究。
목적：연구쌍하탕중불동배비적반하배오하고초대호호파감、뇨밀정제취솔적영향，탐색기변화규률，지도쌍하탕적합리배오。방법：채용Welch Ultimate AQ-C18색보주（4.6 mm×250 mm，5μm），이을정-수위류동상，제도세탈，류속위1.0 mL·min-1，검측파장위265 nm，주온25℃，이용건립적HPLC법측정호호파감、뇨밀정적제취솔，비교득출불동배비대2충성분제취솔적영향。결과：호호파감진양량재0.872-4.36 ng，뇨밀정재10.8-50.4 ng지간선성관계량호，평균가양회수솔분별위96.53%-101.48%， RSD균재0.18%-4.26%。쌍하탕배오후교반하원약호호파감제취솔강저，뇨밀정제취솔증가。결론：복방쌍하탕불동배오대호호파감화뇨밀정적제취솔유일정영향，최가배비치득심입연구。
This article was aimed to study the effect of pinellia combined with prunella vulgarisin of different ratios on the extraction yield of trigonellin and uracil, in order to investigate the changing disciplines and reasonable com-bination of Shuangxia Soup. A Welch Ultimate AQ-C18 column (4.6 mm×250 mm, 5 μm) was used in the study. The mobile phase, consisting of solvent A (water) and solvent B (acetonitrile) was programmed as a linear gradi-ent. The flow rate was 1.0 mL·min-1. And the detection wavelength was 265 nm. The column temperature was 25℃. HPLC was used in the determination of extraction yield of trigonellin and uracil for the comparison among different compatibilities. The results showed that the linearity was achieved in the range of 0.872-4.36 ng for trigonelline, and 10.8-50.4 ng for uracil. The average recoveries were 96.53%-101.48%, with RSD of 0.18%-4.26%. Com-pared with pinellia, the extraction yield of trigonelline was reduced and the extraction yield of uracil was increased after the compatibility. It was concluded that different compatibilities of Shuangxia Soup had effects on the extrac-tion yield of trigonelline and uracil. The best compatibility ratio still requires in-depth study.
