世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
10期
2214-2218
,共5页
谢柳倩%赵灵灵%袁建娜%郑虎占%王灵芝
謝柳倩%趙靈靈%袁建娜%鄭虎佔%王靈芝
사류천%조령령%원건나%정호점%왕령지
蜈蚣%蛋白质%水解%ACE
蜈蚣%蛋白質%水解%ACE
오공%단백질%수해%ACE
centipedes%protein%hydrolysis%ACE
目的：对动物药蜈蚣（Scolopendra subspinipes muitilans L.Koch.）蛋白组分进行测定，并对各组分的酶解物进行血管紧张素转化酶（ACE）体外活性评价。方法：采用顺序抽提法依次进行清蛋白、球蛋白、醇溶蛋白和谷蛋白的提取，用Brandford和凯式定氮法进行蛋白质含量的测定。利用胃蛋白酶水解蜈蚣的4类蛋白及残渣，酶解液超滤冻干得到小分子量多肽（≤3 kD），采用高效液相色谱法对其ACE抑制活性进行测定。结果：蜈蚣总蛋白质含量为（62.69±1.41）%。4大类蛋白及残渣中蛋白占总蛋白含量的百分比分别为：清蛋白（6.42±0.31）%；球蛋白（7.94±0.24）%；醇溶蛋白（4.31±0.34）%；谷蛋白（40.66±0.56）%；残渣（25.78±0.60）%。这4大类蛋白及残渣蛋白酶解物在1 mg·mL-1浓度下的体外ACE抑制率分别为：50.28%、57.37%、31.15%、58.99%、80.81%。结论：蜈蚣中蛋白质含量丰富，且蛋白水解产物均表现出一定的ACE抑制作用，其中残渣蛋白和谷蛋白的ACE抑制作用较强，为蜈蚣临床用药及功能食品的开发提供了理论支持。
目的：對動物藥蜈蚣（Scolopendra subspinipes muitilans L.Koch.）蛋白組分進行測定，併對各組分的酶解物進行血管緊張素轉化酶（ACE）體外活性評價。方法：採用順序抽提法依次進行清蛋白、毬蛋白、醇溶蛋白和穀蛋白的提取，用Brandford和凱式定氮法進行蛋白質含量的測定。利用胃蛋白酶水解蜈蚣的4類蛋白及殘渣，酶解液超濾凍榦得到小分子量多肽（≤3 kD），採用高效液相色譜法對其ACE抑製活性進行測定。結果：蜈蚣總蛋白質含量為（62.69±1.41）%。4大類蛋白及殘渣中蛋白佔總蛋白含量的百分比分彆為：清蛋白（6.42±0.31）%；毬蛋白（7.94±0.24）%；醇溶蛋白（4.31±0.34）%；穀蛋白（40.66±0.56）%；殘渣（25.78±0.60）%。這4大類蛋白及殘渣蛋白酶解物在1 mg·mL-1濃度下的體外ACE抑製率分彆為：50.28%、57.37%、31.15%、58.99%、80.81%。結論：蜈蚣中蛋白質含量豐富，且蛋白水解產物均錶現齣一定的ACE抑製作用，其中殘渣蛋白和穀蛋白的ACE抑製作用較彊，為蜈蚣臨床用藥及功能食品的開髮提供瞭理論支持。
목적：대동물약오공（Scolopendra subspinipes muitilans L.Koch.）단백조분진행측정，병대각조분적매해물진행혈관긴장소전화매（ACE）체외활성평개。방법：채용순서추제법의차진행청단백、구단백、순용단백화곡단백적제취，용Brandford화개식정담법진행단백질함량적측정。이용위단백매수해오공적4류단백급잔사，매해액초려동간득도소분자량다태（≤3 kD），채용고효액상색보법대기ACE억제활성진행측정。결과：오공총단백질함량위（62.69±1.41）%。4대류단백급잔사중단백점총단백함량적백분비분별위：청단백（6.42±0.31）%；구단백（7.94±0.24）%；순용단백（4.31±0.34）%；곡단백（40.66±0.56）%；잔사（25.78±0.60）%。저4대류단백급잔사단백매해물재1 mg·mL-1농도하적체외ACE억제솔분별위：50.28%、57.37%、31.15%、58.99%、80.81%。결론：오공중단백질함량봉부，차단백수해산물균표현출일정적ACE억제작용，기중잔사단백화곡단백적ACE억제작용교강，위오공림상용약급공능식품적개발제공료이론지지。
This study was aimed to analyze the major protein composition of centipedes (Scolopendra subspinipes mutilans L.Koch.), and the ACE inhibitory activity of its hydrolysis. Albumins, gulbulins, coixins and glutelins were sequentially extracted from centipedes flour with corresponding buffer and then quantified by Kjeldahl method and Brandford. Hydrolysation of four kinds of proteins of centipedes and the residues were conducted with pepsin. The hydrolysis was ultrafiltrated (MWCO=3 000) and lyophilized. The peptides (≤3 kD) were obtained to evalu-ate the ACE inhibitory activity by RP-HPLC. The results showed that the total protein content of centipedes was (62.69±1.41)%. Among which the contents of albumins, globulins, coixins, glutelins and residual were account-ing for (6.42±0.31)%, (7.94±0.24)%, (4.31±0.34)%, (40.66±0.56)% and (25.78±0.60)%, respectively. The inhibition rate of hydrolysis of four kinds of protein and residual were 50.28%, 57.37%, 31.15%, 58.99%, 80.81%, respectively. It was concluded that centipedes were rich in protein and the hydrolyzate of all proteins manifested ACE inhibitory activity at different extent. The residual and glutelins indicated strong ACE inhibitory potential by hydrolysis. This research provided valuable sights for exploring hypotensive activity and functional food from centi-pedes.
