CAJ | 학술논문

慢性便秘是一种高发疾病，严重危害人类健康。大黄用于治疗便秘十分有效。其主要成分有大黄游离蒽醌、结合蒽醌、大黄鞣质和大黄多糖，然而哪种成分才是大黄治疗便秘的有效成分却不清楚。本研究提出一种以疾病为基础、以药效为指导的中药有效成分研究方法来进行中药质量控制。首先建立大鼠便秘模型，进行大黄药效成分筛选，进行不同产地药材质量考察，筛选最适产区。并在此基础上，进行大黄提取工艺优化。研究发现，大黄治疗便秘的有效成分是大黄结合蒽醌。与四川平武、北川大黄相比，凉山大黄质量较优。提取工艺为10.34倍乙醇，提取24.25 min，共4次。其最大提取量为5.455 mg·g-1。本研究可为大黄提取工艺质量控制提供参考。
만성편비시일충고발질병，엄중위해인류건강。대황용우치료편비십분유효。기주요성분유대황유리은곤、결합은곤、대황유질화대황다당，연이나충성분재시대황치료편비적유효성분각불청초。본연구제출일충이질병위기출、이약효위지도적중약유효성분연구방법래진행중약질량공제。수선건립대서편비모형，진행대황약효성분사선，진행불동산지약재질량고찰，사선최괄산구。병재차기출상，진행대황제취공예우화。연구발현，대황치료편비적유효성분시대황결합은곤。여사천평무、북천대황상비，량산대황질량교우。제취공예위10.34배을순，제취24.25 min，공4차。기최대제취량위5.455 mg·g-1。본연구가위대황제취공예질량공제제공삼고。
Chronic constipation is a highly debilitating condition, affecting a significant proportion of the commu-nity. Rhei Radix et Rhizoma (rhubarb) is one of the important herbal drugs used in traditional Chinese medicine (TCM) for treating constipation which includes four groups of main active components such as free anthraquinone, conjunc-tive anthraquinone, rhubarb tannin, and rhubarb polysaccharides. However, which one is response for the laxative effect of rhubarb is unknown. Therefore, we proposed a strategy for the effect-guided quality control of TCM based on disease. The effective material for laxative effect of rhubarb was firstly screened based on the constipation model. And different production locations were investigated. Then, the processing was optimized to set up a quality control of rhubarb. It was found that the bioactive component was the conjunctive anthraquinone for the laxative effect. And the raw herb from Liangshan had the best quality compared with those from Pingwu and Beichuan in Sichuan Prov-ince, China. The extraction ethanol volumes to substrate ratio of 10.34, extraction time of 24.25 min and extraction number of 4 were the best conditions for the extraction of conjunctive anthraquinone. Under the optimized condi-tions, the maximum production was 5.455 mg·g-1. This extraction procedure was used as quality control of rhubarb in the treatment of constipation.