CAJ | 학술논문

目的：桂芍等量配伍（1：1），一辛散一酸收，一治卫强一治营弱，解表和里，调和营卫。芍药倍用（1：2），酸甘化阴以滋阴养血。基于桂枝-白芍药对1：1和1：2的中医临床用药特点，探讨桂芍药对不同配比的含量变化。方法：采用液质联用技术，乙腈-0.1%乙酸梯度洗脱，正离子模式，毛细管温度350℃，气化温度300℃，考察桂芍配比1：1和1：2及其单味药水煎液，比较1：1和1：2配比成分及含量差异。结果：桂枝中前花靛B2，2-羟基桂皮醛有明显峰，其中2-羟基桂皮醛在桂枝当量的混煎液中基本无峰，前花靛B2含量在1：1配比高于1：2配比；白芍中白芍酮、芍药苷亚硫酸酯、1，2，3，6-四-O-没食子酰-β-D-葡萄糖、芍药苷、芍药苷同分异构体、苯甲酰芍药苷及苯甲酰芍药苷同分异构体有明显的峰，其中1，2，3，6-四-O-没食子酰-β-D-葡萄糖、芍药苷、苯甲酰芍药苷、苯甲酰芍药苷同分异构体含量1：1配比高于1：2配比，而白芍酮、芍药苷亚硫酸酯、芍药苷同分异构体含量1：2配比高于1：1配比。结论：在桂芍药对不同配比中，前花靛B2、1，2，3，6-四-O-没食子酰-β-D-葡萄糖、芍药苷、苯甲酰芍药苷、苯甲酰芍药苷同分异构体含量1：1配比高于1：2配比，而白芍酮、芍药苷亚硫酸酯、芍药苷同分异构体含量1：2配比高于1：1配比。研究不同配比桂芍药对的成分含量差异，为中医临床辨证论治、合理用药提供支撑。
목적：계작등량배오（1：1），일신산일산수，일치위강일치영약，해표화리，조화영위。작약배용（1：2），산감화음이자음양혈。기우계지-백작약대1：1화1：2적중의림상용약특점，탐토계작약대불동배비적함량변화。방법：채용액질련용기술，을정-0.1%을산제도세탈，정리자모식，모세관온도350℃，기화온도300℃，고찰계작배비1：1화1：2급기단미약수전액，비교1：1화1：2배비성분급함량차이。결과：계지중전화전B2，2-간기계피철유명현봉，기중2-간기계피철재계지당량적혼전액중기본무봉，전화전B2함량재1：1배비고우1：2배비；백작중백작동、작약감아류산지、1，2，3，6-사-O-몰식자선-β-D-포도당、작약감、작약감동분이구체、분갑선작약감급분갑선작약감동분이구체유명현적봉，기중1，2，3，6-사-O-몰식자선-β-D-포도당、작약감、분갑선작약감、분갑선작약감동분이구체함량1：1배비고우1：2배비，이백작동、작약감아류산지、작약감동분이구체함량1：2배비고우1：1배비。결론：재계작약대불동배비중，전화전B2、1，2，3，6-사-O-몰식자선-β-D-포도당、작약감、분갑선작약감、분갑선작약감동분이구체함량1：1배비고우1：2배비，이백작동、작약감아류산지、작약감동분이구체함량1：2배비고우1：1배비。연구불동배비계작약대적성분함량차이，위중의림상변증론치、합리용약제공지탱。
This article was aimed to study the different clinical characteristics using drug pair of Cassia twig and white peony root with the contents ratio of 1:1 and 1:2. Based on the different clinical treatment of drug pair of Cas-sia twig and white peony root, different compositional ingredients in ratio of 1:1 and 1:2 were illuminated by HPLC/MS method. The drug pair of Cassia twig and white peony roots in ratio of 1:1 and 1:2 and single herbs were ex-tracted for HPLC/MS analysis. A protocol was followed, including acetonitrile - 0.1% acetic acid with gradient elution, positive mode, 350℃ capillary temperature and 300℃ vaporization temperature. The results showed that Procyanidol B2 and 2-Hydroxy cinnamal dehyde can be extracted from single Cassia twig, but 2-Hydroxy cinna-mal dehyde cannot be detected in drug pair. It showed the contents of Procyanidol B2 in 1:1 ratio was more than 1:2 ratio. Simultaneously, Palbinone, paeoniflorin sulfonate, 1,2,3,6-Tetra-O-galloyl-β-D-glucose, Paeoniflorin, Pae-oniflorin isomers, Benzoylpaeo-niflorin, and Benzoyl Paeoniflorin isomers can also be dissolved in white peony root. In addition, the contents of 1,2,3,6-Tetra-O-galloyl-β-D-glucose, Paeoniflorin, Benzoylpaeo-niflorin, and Benzoyl Paeoniflorin isomers in 1:1 were more than 1:2. The contents of Palbinone, paeoniflorin sulfonate and Paeoniflorin isomers in 1:2 were more than 1:1. It was concluded that Procyanidol B2, 1,2,3,6-Tetra-O-galloyl-β-D-glucose, Paeoniflorin, Benzoylpaeo-niflorin and Benzoyl Paeoniflorin isomers in 1:1 were more than 1:2. The contents of Pal-binone, Paeoniflorin sulfonate and Paeoniflorin isomers in 1:2 were more than 1:1. It provided a scientific basis for traditional Chinese medicine treatment using rational drug pair.