CAJ | 학술논문

为了初步评价蛹肽蛋白作为大菱鲆新型蛋白源的可行性，本试验测定了水洗或醇洗以及未处理蛹肽蛋白的常规营养成分和氨基酸组成，并与秘鲁红鱼粉进行比较；同时以大菱鲆鱼体氨基酸作为参比，综合运用氨基酸分（AAS）、化学分（CS）、必需氨基酸指数（EAAI）、氨基酸比值系数（RC）和氨基酸比值系数分（SRC）5个指标进行氨基酸平衡性分析。结果显示：蛹肽蛋白粗蛋白质含量均在54%以上，粗脂肪不同处理间差异较大，灰分含量均低于鱼粉。处理后除脯氨酸外，其余氨基酸含量均有所上升。AAS与CS的结果相似，均为鱼粉>水洗>醇洗>未处理。各种处理蛹肽蛋白的第一限制性氨基酸均为含硫氨基酸，其他限制性氨基酸主要是赖氨酸、精氨酸、苏氨酸。蛹肽蛋白EAAI较高，为97.01～97.86，但略低于鱼粉（99.72）；而SRC为77.26~79.18，同样也低于鱼粉（83.10）。综上所述，蛹肽蛋白是一种高蛋白、高能量，氨基酸平衡性较好，有望替代大菱鲆饲料中鱼粉的新型蛋白源。
위료초보평개용태단백작위대릉평신형단백원적가행성，본시험측정료수세혹순세이급미처리용태단백적상규영양성분화안기산조성，병여비로홍어분진행비교；동시이대릉평어체안기산작위삼비，종합운용안기산분（AAS）、화학분（CS）、필수안기산지수（EAAI）、안기산비치계수（RC）화안기산비치계수분（SRC）5개지표진행안기산평형성분석。결과현시：용태단백조단백질함량균재54%이상，조지방불동처리간차이교대，회분함량균저우어분。처리후제포안산외，기여안기산함량균유소상승。AAS여CS적결과상사，균위어분>수세>순세>미처리。각충처리용태단백적제일한제성안기산균위함류안기산，기타한제성안기산주요시뢰안산、정안산、소안산。용태단백EAAI교고，위97.01～97.86，단략저우어분（99.72）；이SRC위77.26~79.18，동양야저우어분（83.10）。종상소술，용태단백시일충고단백、고능량，안기산평형성교호，유망체대대릉평사료중어분적신형단백원。
To evaluate the feasibility of applying fermented silkworm pupae as the new protein source for turbot ,basic nutrition composition and amino acid composition of water-washed,alcohol-washed and untreated silkworm pupae were tested and compared with Peru red fishmeal.Five indexes,i.e. amino acid score (AAS),chemical score (CS),essential amino acid index(EAAI),ratio coefficient(RC),score of ratio coefficient(SRC),were employed to analysis the balance of amino acid with a reference to the whole body amino acid composition of turbot.The results showed that crude protein was over 54%in all types of treatment silkworm pupae;crude lipid varied among treatments;crude ash content was lower than fishmeal.The absolute contents of all amino acids were higher than that after treatment except for proline.AAS and CS showed similar results:fish meal>water-washed>alcohol-washed>untreated.The first limiting amino acid was sulfur-con-taining amino acid with lysine,arginine,and threonine as other limiting amino acids.EAAI was relatively high in silkworm pupae,ranging from 97.01 to 97.96,whereas was slightly lower than that of fishmeal (99.72).Similarly,SRC rangeed be-tween 77.26 and 79.18,and was inferior to fishmeal(83.10).In conclusion,fermented silkworm pupae was a promising sub-stitution for fish meal with high protein,high energy,and well-balanced amino acid composition in the feed of turbot.