CAJ | 학술논문

[目的]研究不同溶剂的假蒟叶提取物对亚硝化反应的影响.[方法]在模拟胃液条件下,测定假蒟叶提取物亚硝酸盐清除率及亚硝胺合成阻断率.[结果]不同溶剂的假蒟提取物均有一定的抑制亚硝化反应作用,其中乙酸乙酯提取物的抑制作用最强,且其对亚硝酸盐的清除率及对亚硝胺合成的阻断率在一定的浓度范围内随其浓度的增加而增加,对亚硝酸盐清除能力较强,其IC50值为0.425mg/ml,对亚硝胺合成阻断的能力相对较低,其IC50值为4.064 mg/ml.[结论]假蒟叶提取物具有较好的亚硝化反应抑制活性且主要通过清除底物亚硝酸盐来实现.
[목적]연구불동용제적가구협제취물대아초화반응적영향.[방법]재모의위액조건하,측정가구협제취물아초산염청제솔급아초알합성조단솔.[결과]불동용제적가구제취물균유일정적억제아초화반응작용,기중을산을지제취물적억제작용최강,차기대아초산염적청제솔급대아초알합성적조단솔재일정적농도범위내수기농도적증가이증가,대아초산염청제능력교강,기IC50치위0.425mg/ml,대아초알합성조단적능력상대교저,기IC50치위4.064 mg/ml.[결론]가구협제취물구유교호적아초화반응억제활성차주요통과청제저물아초산염래실현.
[Objective] To investigate the effect of the extracts of Piper sarmentosum Roxb.in different solvents on the nitrosation reaction.[Method] The nitrite scavenging activity and blocking effect of nitrosamine synthesis were determined under simulated gastric conditions.[Result] The extracts of Piper sarmentosum Roxb.in different solvents exhibit the distinctive activity of nitrite scavenging and nitrosamine synthesis blocking.The fraction of ethyl acetate extract showed the strongest inhibitory activity of nitrosation reaction,scavenging nitrite and blocking nitrosamine formation in a dose-dependent manner.The IC50 value for nitrite scavenging was 0.425 mg/ml,and for nitrosamine synthesis 4.064 mg/ml.[Conclusion] The extracts of Piper sarmentosum Roxb.showed significantly inhibitory activity of nitrosation reaction through nitrite scavenging.