CAJ | 학술논문

[目的]研究离子液体1-乙基-3-甲基咪唑磷酸二乙酯磷酸盐([Emim] DEP)对酿酒酵母SY101生长的影响.[方法]用显微镜观察了不同[Emim]DEP浓度下液体培养基中对数生长期酵母细胞的形态及出芽情况,用分光光度法测得的数值绘制出酵母菌24 h生长曲线.[结果]试验发现,[Emim]DEP对酿酒酵母SY101生长有抑制性,随着[Emim]DEP浓度的增加,抑制作用越明显.当[Emim]DEP浓度高于3 g/L时,酵母菌的形态发生明显改变;浓度高于10 g/L时,酵母菌的出芽率明显下降.通过平板培养测得[Emim]DEP对酵母菌的半有效浓度EC50值为6.67 g/L.[结论]研究可为由离子液体从农林废弃物中制备纳米纤维素后的残渣发酵燃料酒精的研究提供基础数据.
[목적]연구리자액체1-을기-3-갑기미서린산이을지린산염([Emim] DEP)대양주효모SY101생장적영향.[방법]용현미경관찰료불동[Emim]DEP농도하액체배양기중대수생장기효모세포적형태급출아정황,용분광광도법측득적수치회제출효모균24 h생장곡선.[결과]시험발현,[Emim]DEP대양주효모SY101생장유억제성,수착[Emim]DEP농도적증가,억제작용월명현.당[Emim]DEP농도고우3 g/L시,효모균적형태발생명현개변;농도고우10 g/L시,효모균적출아솔명현하강.통과평판배양측득[Emim]DEP대효모균적반유효농도EC50치위6.67 g/L.[결론]연구가위유리자액체종농림폐기물중제비납미섬유소후적잔사발효연료주정적연구제공기출수거.
[Objective] The effects of ionic liquid 1-ethyl-3-methylimidazolium diethylphosphate ([Emim] DEP) on growth activity of Saccharomyces cerevisiae SY101 were studied by signal factor tests.[Method] Observed the morphological structure and budding of Saccharomyces cerevisiae SY101 at Log growth phase in the presence of different concentration of [Emim] DEP.Drawn growth curve according to numerical value by spectrophotography in liquid suspension culture.[Result] The results indicated that[Emim] DEP could inhibit the growth of yeast.The higher the[Emim] DEP concentration,the more obvious the inhibition effect was.The yeast' s single cell morphology changed when[Emim] DEP concentration was greater than 3 g/L,and its reproduction rate by budding decreased when[Emim] DEP concentration was greater than 10 g/L.The half effective concentration (EC50) of[Emim] DEP to Saccharomyces cerevisiae SY101 were measured by using solid and value were 6.67 g/L.[Conclusion] The study can provide basic data for fuel alcohol residue fermentation.