CAJ | 학술논문

目的：研究转化生长因子β1（TGF-β1）及其受体Ⅰ（TGF-βRⅠ）与高碘促成纤维细胞增殖作用的相关性，探索布-加综合征（BCS）隔膜组织形成机制。方法实验分为5组，空白对照组、溶媒组、KI组、TGF-βRⅠ抑制剂（SD-208）组和SD-208和碘化钾（KI）共同作用组。采用CCK-8法检测TGF-βRⅠ抑制剂对高碘培养环境中成纤维细胞增殖率的影响；采用免疫印迹法检测不同浓度（0、250、500、1000、2000、3000μg/L）碘离子对成纤维细胞TGF-β1、TGF-βRⅠ蛋白表达的影响。结果①在1000μg/L碘培养环境中，KI与SD-208共同作用组成纤维细胞增殖率（1.29±0.41）高于SD-208组（0.52±0.10），而低于KI组（1.70±0.03），差异有统计学意义（P<0.05）。②1000μg/L和2000μg/L高碘组成纤维细胞TGF-β1蛋白相对表达量高于其他各组（P<0.05）。各组间成纤维细胞TGF-βRⅠ蛋白相对表达量差异无统计学意义（P>0.05）。结论①高碘因素可能通过提高成纤维细胞TGF-β1蛋白表达而促进成纤维细胞增殖；②高碘导致的成纤维细胞增殖可能与BCS隔膜形成相关。
목적：연구전화생장인자β1（TGF-β1）급기수체Ⅰ（TGF-βRⅠ）여고전촉성섬유세포증식작용적상관성，탐색포-가종합정（BCS）격막조직형성궤제。방법실험분위5조，공백대조조、용매조、KI조、TGF-βRⅠ억제제（SD-208）조화SD-208화전화갑（KI）공동작용조。채용CCK-8법검측TGF-βRⅠ억제제대고전배양배경중성섬유세포증식솔적영향；채용면역인적법검측불동농도（0、250、500、1000、2000、3000μg/L）전리자대성섬유세포TGF-β1、TGF-βRⅠ단백표체적영향。결과①재1000μg/L전배양배경중，KI여SD-208공동작용조성섬유세포증식솔（1.29±0.41）고우SD-208조（0.52±0.10），이저우KI조（1.70±0.03），차이유통계학의의（P<0.05）。②1000μg/L화2000μg/L고전조성섬유세포TGF-β1단백상대표체량고우기타각조（P<0.05）。각조간성섬유세포TGF-βRⅠ단백상대표체량차이무통계학의의（P>0.05）。결론①고전인소가능통과제고성섬유세포TGF-β1단백표체이촉진성섬유세포증식；②고전도치적성섬유세포증식가능여BCS격막형성상관。
Objective To study the relationship between transforming growth factor-β1 (TGF-β1), transforming growth factor-β receptor Ⅰ (TGF-βRⅠ) and high concentration iodine in promoting fibroblast proliferation so as to explore the pathogenesis of the membranous formation in Budd- Chiari syndrome. Methods The experiment included five groups: blank control group, solvent group, KI group, TGF-βRⅠinhibitor group (SD-208) and SD-208 plus KI combination group. ① Fibroblasts were cultured in high content of iodine and treated with TGF-βRI inhibitor then the fibroblast proliferation activity was determined by CCK-8 assy.②The protein expressions of TGF-β1 and TGF-βRⅠof fibroblasts in different concentrations of iodine (0, 250, 500, 1 000, 2 000 and 3 000 ug/L) were determined by Western-blot method. Results ①When the culture solution was of 1 000 ug/L iodine concentration, the cell proliferation rate of the SD-208 plus KI combination group (A:1.29 ± 0.41) was significantly higher than that of the control group (0.52 ± 0.10), but significantly lower than that of the KI group(1.70 ± 0.03) with P < 0.05. ② Fibroblast TGF-β1 protein relative expression levels in the groups with the iodine concentration of 1 000 ug/L and 2 000 ug/L were significantly higher than those of the other groups (P < 0.05). No significant difference in fibroblast TGF-βRⅠ protein relative expressions existed between each other groups (P > 0.05). Conclusion ①High concentration of iodine may promote the proliferation of fibroblasts through raising TGF - β1 protein expression. ② The proliferation of fibroblasts caused by high concentration of iodine may be related to the membranous formation in Budd-Chiari syndrome.