国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
5期
438-441
,共4页
刘振中%刘安飞%王忠红%冯华强%李阳友
劉振中%劉安飛%王忠紅%馮華彊%李暘友
류진중%류안비%왕충홍%풍화강%리양우
氢化可的松%小鼠%阳虚%模型%指标
氫化可的鬆%小鼠%暘虛%模型%指標
경화가적송%소서%양허%모형%지표
Hydrocortisone%Mice%Yang deficiency%Model%Index
目的：比较和分析氢化可的松不同给药途径、剂量致小鼠阳虚模型相关指标的变化。方法以肌肉和腹腔注射两种途径给予小鼠高、低两种剂量（12.5 mg/kg、25 mg/kg氢化可的松制作阳虚模型，观察模型小鼠的症状、体质量、平均摄食量、应激指标以及性器官和免疫器官的脏器系数的改变。结果①腹腔注射给药途径，高剂量模型组的体质量（24.52±3.29）g、体温（35.24±0.32）℃明显低于对照组（31.10±6.11）g、（37.02±0.64）℃；低剂量模型组的平均摄食量（4.30±0.29）g/(只?d)明显低于对照组（7.38±0.53）g/(只?d)。低剂量模型组的包皮腺系数（0.10±0.02）、阴茎系数（0.15±0.03）、胸腺系数（0.12±0.03）均低于对照组（0.15±0.02、0.18±0.02、0.22±0.08、0.98±0.45）。②肌肉注射给药：高剂量模型组平均摄食量（5.92±2.01）g/(只?d)，明显低于对照组（8.60±1.33）g/(只?d)；低剂量模型组体温（34.90±0.22）℃、低温游泳时间（34.00±22.41）s均低于对照组（36.43±0.91）℃、（67.17±21.93）s。两个模型组的胸腺系数（0.10±0.02）、（0.11±0.06）均低于对照组（0.17±0.00）、（0.25±0.03）。结论不同途径、不同剂量的氢化可的松给药致小鼠阳虚症状出现的时间、程度和相关敏感指标不同。
目的：比較和分析氫化可的鬆不同給藥途徑、劑量緻小鼠暘虛模型相關指標的變化。方法以肌肉和腹腔註射兩種途徑給予小鼠高、低兩種劑量（12.5 mg/kg、25 mg/kg氫化可的鬆製作暘虛模型，觀察模型小鼠的癥狀、體質量、平均攝食量、應激指標以及性器官和免疫器官的髒器繫數的改變。結果①腹腔註射給藥途徑，高劑量模型組的體質量（24.52±3.29）g、體溫（35.24±0.32）℃明顯低于對照組（31.10±6.11）g、（37.02±0.64）℃；低劑量模型組的平均攝食量（4.30±0.29）g/(隻?d)明顯低于對照組（7.38±0.53）g/(隻?d)。低劑量模型組的包皮腺繫數（0.10±0.02）、陰莖繫數（0.15±0.03）、胸腺繫數（0.12±0.03）均低于對照組（0.15±0.02、0.18±0.02、0.22±0.08、0.98±0.45）。②肌肉註射給藥：高劑量模型組平均攝食量（5.92±2.01）g/(隻?d)，明顯低于對照組（8.60±1.33）g/(隻?d)；低劑量模型組體溫（34.90±0.22）℃、低溫遊泳時間（34.00±22.41）s均低于對照組（36.43±0.91）℃、（67.17±21.93）s。兩箇模型組的胸腺繫數（0.10±0.02）、（0.11±0.06）均低于對照組（0.17±0.00）、（0.25±0.03）。結論不同途徑、不同劑量的氫化可的鬆給藥緻小鼠暘虛癥狀齣現的時間、程度和相關敏感指標不同。
목적：비교화분석경화가적송불동급약도경、제량치소서양허모형상관지표적변화。방법이기육화복강주사량충도경급여소서고、저량충제량（12.5 mg/kg、25 mg/kg경화가적송제작양허모형，관찰모형소서적증상、체질량、평균섭식량、응격지표이급성기관화면역기관적장기계수적개변。결과①복강주사급약도경，고제량모형조적체질량（24.52±3.29）g、체온（35.24±0.32）℃명현저우대조조（31.10±6.11）g、（37.02±0.64）℃；저제량모형조적평균섭식량（4.30±0.29）g/(지?d)명현저우대조조（7.38±0.53）g/(지?d)。저제량모형조적포피선계수（0.10±0.02）、음경계수（0.15±0.03）、흉선계수（0.12±0.03）균저우대조조（0.15±0.02、0.18±0.02、0.22±0.08、0.98±0.45）。②기육주사급약：고제량모형조평균섭식량（5.92±2.01）g/(지?d)，명현저우대조조（8.60±1.33）g/(지?d)；저제량모형조체온（34.90±0.22）℃、저온유영시간（34.00±22.41）s균저우대조조（36.43±0.91）℃、（67.17±21.93）s。량개모형조적흉선계수（0.10±0.02）、（0.11±0.06）균저우대조조（0.17±0.00）、（0.25±0.03）。결론불동도경、불동제량적경화가적송급약치소서양허증상출현적시간、정도화상관민감지표불동。
Objective To compare and analyze the index variation of Yang deficiency mice induced by hydrocortisone in different way of administration and dose. Methods The mice model of Yang deficiency were induced by low dose and high dose hydrocortisone(12.5 mg/kg, 25 mg/kg) in two ways of intramuscular and intraperitoneal injection. The symptoms, body weight, the average intake, the index of stress and the coefficient of sex organs and immune organs of animals were observed. Results In the way of intraperitoneal injection, the weight(24.52±3.29)g, body temperature(35.24±0.32)℃ of high dose of model group were significantly lower than that of control group(31.10±6.11)g,(37.02±0.64)℃. The average intake of low dose group(4.30 ± 0.29)g/(per?d) was lower than control group(7.38±0.53)g/(per?d), and the coefficient of preputial glands(0.10±0.02), penis(0.15±0.03), thymus(0.12±0.03)were lower than that of control group. In the way of intramuscular injection, the average intake of high dose group(5.92±2.01)g/(per?d) was lower than control group(8.60±1.33)g/(per?d). The body temperature(34.90±0.22)℃ and the time of swimming in low temperature (34.00±22.41)s of low dose model group were lower than that of control group(36.43±0.91)℃, (67.17±21.93)s, and the coefficient of thymus of two model groups(0.10±0.02),(0.11±0.06)were lower than that of control group. Conclusion Various dose and model establishment methods of Yang deficiency mice have different the time of symptom appearing, the degree of symptom and sensitive index.
