CAJ | 학술논문

目的：研究平喘方对哮喘模型小鼠Rho/Rock信号通路表达的影响，探讨平喘方治疗哮喘的机理。方法采用随机数字表法将40只BALB/C小鼠随机分为空白对照组、哮喘模型组、地塞米松组和平喘方干预组各10只。给药干预4周后用Western blot法检测各组小鼠肺组织RhoA蛋白、RockI蛋白表达量，RT-PCR法检测小鼠肺组织RhoA mRNA、RockI mRNA表达量。结果哮喘模型组小鼠RhoA蛋白、RockI蛋白、RhoA mRNA、RockI mRNA表达量[分别为（1.05±0.20）、（1.06±0.08）、（6.60±1.09）、（6.53±1.84）]，均高于空白对照组[分别为（0.76±0.08）、（0.84±0.14）、（3.82±1.77）、（3.65±1.46）]，差异有统计学意义（P＜0.01）。地塞米松干预组小鼠RhoA蛋白、RockI蛋白、RhoA mRNA、RockI mRNA表达量[分别为（0.78±0.11）、（0.87±0.32）、（4.19±2.33）、（4.09±1.08）]，低于哮喘模型组（P＜0.01）。平喘方干预组[分别为（0.86±0.12）、（0.93±0.14）、（4.38±2.01）、（4.50±1.13）]低于哮喘模型组，差异有统计学意义（P＜0.05）；平喘方干预组与地塞米松干预组比较差异无统计学意义（P＞0.05）。结论平喘方可抑制哮喘小鼠Rho/Rock信号通路表达，效果与地塞米松相近。
목적：연구평천방대효천모형소서Rho/Rock신호통로표체적영향，탐토평천방치료효천적궤리。방법채용수궤수자표법장40지BALB/C소서수궤분위공백대조조、효천모형조、지새미송조화평천방간예조각10지。급약간예4주후용Western blot법검측각조소서폐조직RhoA단백、RockI단백표체량，RT-PCR법검측소서폐조직RhoA mRNA、RockI mRNA표체량。결과효천모형조소서RhoA단백、RockI단백、RhoA mRNA、RockI mRNA표체량[분별위（1.05±0.20）、（1.06±0.08）、（6.60±1.09）、（6.53±1.84）]，균고우공백대조조[분별위（0.76±0.08）、（0.84±0.14）、（3.82±1.77）、（3.65±1.46）]，차이유통계학의의（P＜0.01）。지새미송간예조소서RhoA단백、RockI단백、RhoA mRNA、RockI mRNA표체량[분별위（0.78±0.11）、（0.87±0.32）、（4.19±2.33）、（4.09±1.08）]，저우효천모형조（P＜0.01）。평천방간예조[분별위（0.86±0.12）、（0.93±0.14）、（4.38±2.01）、（4.50±1.13）]저우효천모형조，차이유통계학의의（P＜0.05）；평천방간예조여지새미송간예조비교차이무통계학의의（P＞0.05）。결론평천방가억제효천소서Rho/Rock신호통로표체，효과여지새미송상근。
Objective To study the effect of Pingchuan Formula(PCF)on Rho/Rock signaling pathway in the mice of asthmatic model, explore the mechanism of PCF on asthma. Methods Forty BALB/C mice were randomly divided into four groups:a control group, a model group,a dexamethasone group and a PCF group. After treated with medicine for 4 weeks, albumen expression of RhoA and RockI in lung tissues were detected by using Western blot, mRNA expressions of RhoA and RockI were detected by using Real-time PCR. Results The albumen and the mRNA expression of RhoA and RockI in the model group were(1.05± 0.20),(1.06±0.08),(6.60±1.09), (6.53±1.84), the control group were(0.76±0.08), (0.84±0.14), (3.82± 1.77), (3.65±1.46),there was a significant difference between two groups(P<0.01). The albumen and the mRNA expression of RhoA and RockI in the dexamethasone group were(0.78±0.11), (0.87±0.32), (4.19± 2.33), (4.09±1.08), there was a significant difference compared with the model group(P<0.01). The PCF group were(0.86±0.12), (0.93±0.14), (4.38±2.01), (4.50±1.13), there was a difference compared with the model group(P<0.05). These was no difference between the dexamethasone group and the PCF group(P>0.05). Conclusion The expressions of Rho/Rock signaling pathway were controlled significantly by PCF, and the curative effect was similar to dexamethasone.