CAJ | 학술논문

目的：从12种具有活血化瘀功能的中草药饮片中分离筛选出具有纤溶酶活性的成分。方法采用水浸盐析的方法从12种中草药饮片中得到蛋白粗提物，并采用纤维蛋白平板法对其进行活性测定，制作尿激酶标准曲线测定提取物中纤溶酶成分的比活力。结果其中11种中草药蛋白粗提物中含有纤溶酶成分，36 h后瓜蒌产生的溶圈面积最大，泽泻和益母草次之，而黄芪产生的溶圈面积最小。综合蛋白质浓度、纤维蛋白平板上提取物的溶圈面积以及蛋白质比活力，发现当归提取物的纤溶酶比活力最大，为48.46 U/mg。结论12种中草药饮片的蛋白粗提物除桃仁外均具有纤溶酶活性，对纤维蛋白具有不同程度的溶解作用。
목적：종12충구유활혈화어공능적중초약음편중분리사선출구유섬용매활성적성분。방법채용수침염석적방법종12충중초약음편중득도단백조제물，병채용섬유단백평판법대기진행활성측정，제작뇨격매표준곡선측정제취물중섬용매성분적비활력。결과기중11충중초약단백조제물중함유섬용매성분，36 h후과루산생적용권면적최대，택사화익모초차지，이황기산생적용권면적최소。종합단백질농도、섬유단백평판상제취물적용권면적이급단백질비활력，발현당귀제취물적섬용매비활력최대，위48.46 U/mg。결론12충중초약음편적단백조제물제도인외균구유섬용매활성，대섬유단백구유불동정도적용해작용。
Objective To separate and screen components with fibrinolytic enzyme activity from twelve Chinese herbal medicines. Methods The components were extracted with water and precipitated with salt, and they were tested by fibrinolytic protein plates method. The active components with fibrinolytic activity were separated and screened which were compared with urokinase. Results Eleven of the twelve extracts showed fibrinolytic activity, while Trichosanthes kirilowii got the biggest fibrinolytic zone after 36 hours, followed by Alisma plantago-aquatica and Leonurus japonicus, and the Radix Astagali got the smallest one. According to the concentration of the protein, the area of the fibrinolytic zone and the specific activity of the components, the extract from Angelica sinensis exhibited the best specific activity at level of 48.46U/mg. Conclusion The extracts from Chinese herbal medicines except Semen Persicae exhibit fibrinolytic enzyme activity which can dissolve the fibrin in different degrees.