CAJ | 학술논문

目的：探讨宁动颗粒对抽动-秽语综合征（TS）模型大鼠纹状体5-羟色胺（5-HT）代谢及其受体5-HT2A受体（5-HT2AR）的影响及其作用机制。方法将64只大鼠按照随机数字表法随机分为空白对照组、模型组、宁动颗粒低剂量组、宁动颗粒高剂量组各16只，通过腹腔注射阿朴吗啡（Apo）建立TS模型。宁动颗粒低、高剂量组分别给予宁动颗粒1.8、3.6 g/(kg?d)；正常对照组及模型组分别给予等量蒸馏水。药物干预12周后，取大鼠纹状体，采用免疫组化法检测纹状体5HT2AR表达，酶联免疫吸附法测定大鼠纹状体5-HT、5-羟吲哚乙酸(5-HIAA)含量。结果模型组大鼠纹状体5-HT、5-HT2AR蛋白表达升高[分别为（1.98±0.14）ng/ml、（143.10±8.01）]，与空白对照组[（1.78±0.10）ng/ml、（127.43±6.72）]比较，差异有统计学意义（P＜0.01或0.05）。宁动颗粒高剂量可上调大鼠纹状体5-HT、5-HT2AR蛋白表达[分别为（2.20±0.14）ng/ml、（166.33±9.21）]，下调5-HIAA表达[（0.58±0.08）ng/ml]，与模型组比较差异有统计学意义（P＜0.01或0.05）。结论 Apo诱导的TS大鼠纹状体存在5-HT递质系统功能增强，但增强功能较弱；宁动颗粒可抑制5-HT代谢，上调TS大鼠纹状体内5-HT2AR蛋白表达和5-HT含量，改善纹状体内5-HT递质系统功能活动的相对不足。
목적：탐토저동과립대추동-예어종합정（TS）모형대서문상체5-간색알（5-HT）대사급기수체5-HT2A수체（5-HT2AR）적영향급기작용궤제。방법장64지대서안조수궤수자표법수궤분위공백대조조、모형조、저동과립저제량조、저동과립고제량조각16지，통과복강주사아박마배（Apo）건립TS모형。저동과립저、고제량조분별급여저동과립1.8、3.6 g/(kg?d)；정상대조조급모형조분별급여등량증류수。약물간예12주후，취대서문상체，채용면역조화법검측문상체5HT2AR표체，매련면역흡부법측정대서문상체5-HT、5-간신타을산(5-HIAA)함량。결과모형조대서문상체5-HT、5-HT2AR단백표체승고[분별위（1.98±0.14）ng/ml、（143.10±8.01）]，여공백대조조[（1.78±0.10）ng/ml、（127.43±6.72）]비교，차이유통계학의의（P＜0.01혹0.05）。저동과립고제량가상조대서문상체5-HT、5-HT2AR단백표체[분별위（2.20±0.14）ng/ml、（166.33±9.21）]，하조5-HIAA표체[（0.58±0.08）ng/ml]，여모형조비교차이유통계학의의（P＜0.01혹0.05）。결론 Apo유도적TS대서문상체존재5-HT체질계통공능증강，단증강공능교약；저동과립가억제5-HT대사，상조TS대서문상체내5-HT2AR단백표체화5-HT함량，개선문상체내5-HT체질계통공능활동적상대불족。
Objective To investigate the effect of Ningdong granule on 5-hydroxytryptamine metabolism and 5-HT2AR of Tourette’s Syndrome(TS) rat models. Methods 32 SD rats were divided into 4 groups randomly, the control group, the model (Apo) group, the low dosage NDG (NGL) group, and the high dosage (NGH) group. TS rat models were induced by intraperitoneal injection (i.p.) with Apo (2 mg/kg) in the experimental groups last 4 weeks. Rats in the control group were injected with normal saline (0.9%) (3 ml/kg, i.p.). Then, the rats were treated by intragastric administration (i.g.) with ND granular at 1.8g/(kg?d)in NGL group, with ND granular at 3.6 g/(kg?d)in NGH group, with distilled water at equal Volume in Control group and Apo group respectively for 12 successive weeks. Analysis the striatum 5-HT2AR protein expression by Immunohistochemical method;Determination of the content of 5-HT and 5-HIAA in striatum by Enzyme linked immunosorbent assay (ELISA). Results The level of 5-HT and 5-HT2AR protein expression in the model group [(1.98±0.14)ng/ml and(143.10±8.01)], compared with the control group[(1.78±0.10)ng/ml and(127.43±6.72)], was up-regulated (P<0.01 or 0.05). The levels of 5-HT and 5-HT2AR protein expression in NGH group[(2.20±0.14)ng/ml and(166.33±9.21)ng/ml] were further up-regulated, and the levels of 5-HIAA in NDH group [(0.58±0.08) ng/ml] became lower, compared with the control group(P<0.01 or 0.05). Conclusion Activity of 5-HT system enhanced in Apo-induced TS rat model. However, the enhanced activity may still be relatively insufficient. The mechanism of ND granulear treating TS was associated with depression of the 5-HT metabolism, up-regulation expression of 5-HT2AR, and improving the activity insufficient of 5-HT system.