国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
6期
535-538
,共4页
禹玉洪%张国伟%杨晓宁%李燕舞%巫燕莉%王汝俊
禹玉洪%張國偉%楊曉寧%李燕舞%巫燕莉%王汝俊
우옥홍%장국위%양효저%리연무%무연리%왕여준
丁桂儿脐贴%地芬诺酯%罗通定%吲哚美辛%止泻%镇痛
丁桂兒臍貼%地芬諾酯%囉通定%吲哚美辛%止瀉%鎮痛
정계인제첩%지분낙지%라통정%신타미신%지사%진통
Dingguier paste%Diphenoxylate%Rotundin%Indomethacin%Antidiarrheal%Analgesia
目的：观察丁桂儿脐贴联合用药及丁桂儿口服用药对腹泻模型小鼠的止泻、镇痛作用。方法采用番泻叶小鼠腹泻模型、蓖麻油小鼠腹泻模型，按体质量随机分为模型组（10只）、丁桂儿赋性剂组（10只）、丁桂儿脐贴小（10只）、中（11只）、大剂量联合阳性药（11只）、阳性药组（11只）及丁桂儿口服组（11只），观察丁桂儿脐贴联合用药及丁桂儿口服后对腹泻模型的止泻作用。采用热板法、醋酸法，按正常对照组、丁桂儿脐贴小、中、大剂量联合阳性药、阳性药组及丁桂儿口服组给药，观察丁桂儿脐贴联合用药、丁桂儿口服后的镇痛作用。结果①丁桂儿脐贴小、中、大剂量＋地芬诺酯组、丁桂儿脐贴小剂量＋补脾益肠丸组、地芬诺酯组、补脾益肠丸组、丁桂儿口服组均可减少番泻叶小鼠腹泻模型2 h内稀便粒数[分别为（0.50±1.27）个、（2.27±2.90）个、（1.18±1.83）个，（2.00±2.26）个、（0.73±1.56）个、（1.91±2.95）个、（2.55±2.11）个]；丁桂儿脐贴小、大剂量＋地芬诺酯组可显著减少番泻叶小鼠腹泻模型4h内稀便粒数[分别为（6.70±2.11）个、（6.27±3.20）个]；丁桂儿脐贴大剂量＋地芬诺酯组可显著减少番泻叶小鼠腹泻模型6h内稀便粒数[（6.91±2.77）个]。②丁桂儿脐贴小剂量＋地芬诺酯组可减少蓖麻油小鼠腹泻模型2 h、4 h、6 h内稀便粒数[分别为（1.10±1.29）个、（3.20±0.63）个、（4.60±2.07）个]，丁桂儿脐贴中剂量＋地芬诺酯组可减少蓖麻油小鼠腹泻模型6 h稀便粒数[（4.64±2.45）个]，丁桂儿口服组可显著减少蓖麻油小鼠腹泻模型2 h、4 h、6 h内稀便粒数[分别为（0.45±0.82）个、（2.91±2.07）个、（4.27±2.15）个]。③丁桂儿脐贴小、中、大剂量＋罗通定组可提高小鼠给药后30 min、60 min、90 min、120 min痛阈[小剂量依次为（52.32±17.29）s、（56.24±12.48）s、（57.28±6.61）s、（58.58±4.70）s，中剂量依次为（48.27±19.35）s、（54.92±12.59）s、（55.91±11.48）s、（55.15±12.49）s，大剂量依次为（49.15±15.69）s、（56.24±11.89）s、（59.08±2.93）s]。④丁桂儿脐贴中剂量＋吲哚美辛组可明显延长乙酸刺激引起的疼痛潜伏期（14.72±5.99）min，丁桂儿脐贴小、中、大剂量＋吲哚美辛各组可显著减少乙酸刺激引起的小鼠扭体次数[分别为（7.62±8.31）次、（3.62±6.14）次、（11.25±9.46）次]。结论丁桂儿口服、丁桂儿脐贴联合用药有止泻、镇痛作用。
目的：觀察丁桂兒臍貼聯閤用藥及丁桂兒口服用藥對腹瀉模型小鼠的止瀉、鎮痛作用。方法採用番瀉葉小鼠腹瀉模型、蓖痳油小鼠腹瀉模型，按體質量隨機分為模型組（10隻）、丁桂兒賦性劑組（10隻）、丁桂兒臍貼小（10隻）、中（11隻）、大劑量聯閤暘性藥（11隻）、暘性藥組（11隻）及丁桂兒口服組（11隻），觀察丁桂兒臍貼聯閤用藥及丁桂兒口服後對腹瀉模型的止瀉作用。採用熱闆法、醋痠法，按正常對照組、丁桂兒臍貼小、中、大劑量聯閤暘性藥、暘性藥組及丁桂兒口服組給藥，觀察丁桂兒臍貼聯閤用藥、丁桂兒口服後的鎮痛作用。結果①丁桂兒臍貼小、中、大劑量＋地芬諾酯組、丁桂兒臍貼小劑量＋補脾益腸汍組、地芬諾酯組、補脾益腸汍組、丁桂兒口服組均可減少番瀉葉小鼠腹瀉模型2 h內稀便粒數[分彆為（0.50±1.27）箇、（2.27±2.90）箇、（1.18±1.83）箇，（2.00±2.26）箇、（0.73±1.56）箇、（1.91±2.95）箇、（2.55±2.11）箇]；丁桂兒臍貼小、大劑量＋地芬諾酯組可顯著減少番瀉葉小鼠腹瀉模型4h內稀便粒數[分彆為（6.70±2.11）箇、（6.27±3.20）箇]；丁桂兒臍貼大劑量＋地芬諾酯組可顯著減少番瀉葉小鼠腹瀉模型6h內稀便粒數[（6.91±2.77）箇]。②丁桂兒臍貼小劑量＋地芬諾酯組可減少蓖痳油小鼠腹瀉模型2 h、4 h、6 h內稀便粒數[分彆為（1.10±1.29）箇、（3.20±0.63）箇、（4.60±2.07）箇]，丁桂兒臍貼中劑量＋地芬諾酯組可減少蓖痳油小鼠腹瀉模型6 h稀便粒數[（4.64±2.45）箇]，丁桂兒口服組可顯著減少蓖痳油小鼠腹瀉模型2 h、4 h、6 h內稀便粒數[分彆為（0.45±0.82）箇、（2.91±2.07）箇、（4.27±2.15）箇]。③丁桂兒臍貼小、中、大劑量＋囉通定組可提高小鼠給藥後30 min、60 min、90 min、120 min痛閾[小劑量依次為（52.32±17.29）s、（56.24±12.48）s、（57.28±6.61）s、（58.58±4.70）s，中劑量依次為（48.27±19.35）s、（54.92±12.59）s、（55.91±11.48）s、（55.15±12.49）s，大劑量依次為（49.15±15.69）s、（56.24±11.89）s、（59.08±2.93）s]。④丁桂兒臍貼中劑量＋吲哚美辛組可明顯延長乙痠刺激引起的疼痛潛伏期（14.72±5.99）min，丁桂兒臍貼小、中、大劑量＋吲哚美辛各組可顯著減少乙痠刺激引起的小鼠扭體次數[分彆為（7.62±8.31）次、（3.62±6.14）次、（11.25±9.46）次]。結論丁桂兒口服、丁桂兒臍貼聯閤用藥有止瀉、鎮痛作用。
목적：관찰정계인제첩연합용약급정계인구복용약대복사모형소서적지사、진통작용。방법채용번사협소서복사모형、비마유소서복사모형，안체질량수궤분위모형조（10지）、정계인부성제조（10지）、정계인제첩소（10지）、중（11지）、대제량연합양성약（11지）、양성약조（11지）급정계인구복조（11지），관찰정계인제첩연합용약급정계인구복후대복사모형적지사작용。채용열판법、작산법，안정상대조조、정계인제첩소、중、대제량연합양성약、양성약조급정계인구복조급약，관찰정계인제첩연합용약、정계인구복후적진통작용。결과①정계인제첩소、중、대제량＋지분낙지조、정계인제첩소제량＋보비익장환조、지분낙지조、보비익장환조、정계인구복조균가감소번사협소서복사모형2 h내희편립수[분별위（0.50±1.27）개、（2.27±2.90）개、（1.18±1.83）개，（2.00±2.26）개、（0.73±1.56）개、（1.91±2.95）개、（2.55±2.11）개]；정계인제첩소、대제량＋지분낙지조가현저감소번사협소서복사모형4h내희편립수[분별위（6.70±2.11）개、（6.27±3.20）개]；정계인제첩대제량＋지분낙지조가현저감소번사협소서복사모형6h내희편립수[（6.91±2.77）개]。②정계인제첩소제량＋지분낙지조가감소비마유소서복사모형2 h、4 h、6 h내희편립수[분별위（1.10±1.29）개、（3.20±0.63）개、（4.60±2.07）개]，정계인제첩중제량＋지분낙지조가감소비마유소서복사모형6 h희편립수[（4.64±2.45）개]，정계인구복조가현저감소비마유소서복사모형2 h、4 h、6 h내희편립수[분별위（0.45±0.82）개、（2.91±2.07）개、（4.27±2.15）개]。③정계인제첩소、중、대제량＋라통정조가제고소서급약후30 min、60 min、90 min、120 min통역[소제량의차위（52.32±17.29）s、（56.24±12.48）s、（57.28±6.61）s、（58.58±4.70）s，중제량의차위（48.27±19.35）s、（54.92±12.59）s、（55.91±11.48）s、（55.15±12.49）s，대제량의차위（49.15±15.69）s、（56.24±11.89）s、（59.08±2.93）s]。④정계인제첩중제량＋신타미신조가명현연장을산자격인기적동통잠복기（14.72±5.99）min，정계인제첩소、중、대제량＋신타미신각조가현저감소을산자격인기적소서뉴체차수[분별위（7.62±8.31）차、（3.62±6.14）차、（11.25±9.46）차]。결론정계인구복、정계인제첩연합용약유지사、진통작용。
Objective To observe the antidiarrheal and analgesic effects of Dingguier paste on the mouse. Methods Tho diarrhea mouse models were made by senna leaf or castor oil to detect the antidiarrheal effect of Dingguier paste combination with other drugs. The hot plate test and acetic acid test were used to observe the analgesic effect of Dingguier oral medicine and Dingguier paste in combined use with other drugs. Results Compared with senna leaf model group, Dingguier paste low-dose, middle-dose, high-dose combined with diphenoxylate, Bupi-Yichang pill and Dingguier oral medicine group could decrease the number of feces in 2 h [Number of each group feces was(0.50±1.27), (2.27±2.90), (1.18±1.83);(2.00±2.26), (3.40±2.17), (3.82±1.72);(0.73±1.56), (1.91±2.95), (2.55±2.11)]. Dingguier paste low-dose and high-dose combined with diphenoxylate could decrease the number of feces in 4h [Number of each group feces was (6.70±2.11), (6.27±3.20)]. Dingguier oral group decrease the number of feces in 6h [Number of feces was (6.91±2.77)]. Compared with castor oil model group, Dingguier paste low-dose combined with diphenoxylate could obviously decrease the number of feces in 2 h, 4 h and 6 h [Number of each group feces was(0.45±0.82), (0.45±0.82), (4.27±2.15)]. Dingguier paste middle-dose combined with diphenoxylate could decrease the number of feces in 6 h [Number of feces was (4.64±2.45)]. Dingguier oral group decrease the number of feces in 2 h, 4 h, 6 h [Number of each group feces was (0.45±0.82), (2.91±2.07), (4.27±2.15)].Compared with normal group, the threshold of pain stimulated by hot plate of the Dingguier paste each dose combined with rotundin could increased remarkable after 30 min, 60 min, 90 min and 120 min[The time of Dingguier paste low-dose was(52.32 ± 17.29)s, (56.24 ± 12.48)s, (57.28 ± 6.61)s, (58.58 ± 4.70)s; the time of Dingguier paste middle-dose was (48.27±19.35)s, (54.92±12.59)s, (55.91±11.48)s, (55.15±12.49)s;the time of Dingguier paste high-dose was (49.15±15.69)s, (56.24±11.89)s, 60 s is(59.08±2.93)s]. Compared with normal group, the eclipse time of pain irritated by acetic acid of the Dingguier paste middle-dose combined with indomethacin could increased remarkable(14.72±5.99)min, the writhing frequency could decrease of the Dingguier paste each dose combined with indomethacin[Number of each dose writhing frequency was (7.62±8.31), (3.62± 6.14), (11.25±9.46)]. Conclusion Dingguier paste in the combination use with other drugs and Dingguier oral medicine may have some antidiarrheal and analgesia effects.
