CAJ | 학술논문

目的：介绍一种快速建立的继发性脾功能亢进（脾亢）的猪动物模型，并在此模型基础上进行射频消融治疗（RFA）研究。方法在B型超声或CT导向下经皮穿刺猪脾静脉主干并栓塞引起淤血性脾肿大，再进行RFA治疗，观察动物外周血细胞变化，了解脾脏影像学变化以及组织病理学改变。结果脾静脉栓塞后1周外周血红细胞、血小板即下降，第3周以后二者下降明显（P＜0.05），红细胞和血小板减少，脾肿大可持续6周以上。术后第2周起，脾脏组织学改变符合脾淤血改变。射频热能引起脾脏局部凝固坏死，脾内血管闭塞、广泛微血栓导致血栓性梗死形成。结论经皮脾静脉栓塞建立继发性脾亢方法简单、确切，可以作为脾亢外科或介入治疗的较理想模型，RFA治疗是一种有效的干预措施。
목적：개소일충쾌속건립적계발성비공능항진（비항）적저동물모형，병재차모형기출상진행사빈소융치료（RFA）연구。방법재B형초성혹CT도향하경피천자저비정맥주간병전새인기어혈성비종대，재진행RFA치료，관찰동물외주혈세포변화，료해비장영상학변화이급조직병이학개변。결과비정맥전새후1주외주혈홍세포、혈소판즉하강，제3주이후이자하강명현（P＜0.05），홍세포화혈소판감소，비종대가지속6주이상。술후제2주기，비장조직학개변부합비어혈개변。사빈열능인기비장국부응고배사，비내혈관폐새、엄범미혈전도치혈전성경사형성。결론경피비정맥전새건립계발성비항방법간단、학절，가이작위비항외과혹개입치료적교이상모형，RFA치료시일충유효적간예조시。
Objective To introduce a method for fast establishment of pig models with secondary hypersplenism, and using this model to study the effect of radiofrequency ablation (RFA). Methods Congestive splenomegaly was created by CT- guided percutaneous embolization of splenic vein and its collateral branches in pigs. Then radiofrequency ablation was employed in the models to observe the changes of peripheral blood as well as the spleen changes in its morphology and histopathology. The blood cell count for peripheral venous blood was determined once a week, and the spleen size was measured by CT scanning. Results One week after splenic vein embolization, the counts of peripheral erythrocytes and platelets showed a reduction, which became more obvious in three weeks (P < 0.05). Erythropenia, thrombocytopenia and splenomegaly could last for over six weeks. At the second week after the procedure , pathologically the spleen assumed passive congestion appearance. The thermal energy produced by radiofrequency ablation could cause local splenic coagulation necrosis, splenic vessels occlusion and splenic thrombotic infarction due to diffuse micro-vascular thrombosis. Conclusion To establish an animal model with secondary hypersplenism, the method of CT-guided percutaneous embolism of splenic vein is simple and reliable. The animal models can be used for surgical or interventional researches. Radiofrequency ablation is an effective intervention means.