CAJ | 학술논문

目的：建立测定二陈合剂中橙皮苷含量的方法。方法采用高效液相色谱法，色谱柱为Fortis C18（250 mm×4.6 mm，5μm），流动相为乙腈-醋酸-水（22∶4∶74），流速为1.0 ml/min，柱温30℃，检测波长为283 nm。结果橙皮苷在2.86～57.20μg（r＝0.9998）范围有良好的线性关系，平均加样回收率为99.4%；RSD小于2.0%。结论本方法快速、可靠、简便，重复性好，专属性强，可为二陈合剂的质量标准提供参考。
목적：건립측정이진합제중등피감함량적방법。방법채용고효액상색보법，색보주위Fortis C18（250 mm×4.6 mm，5μm），류동상위을정-작산-수（22∶4∶74），류속위1.0 ml/min，주온30℃，검측파장위283 nm。결과등피감재2.86～57.20μg（r＝0.9998）범위유량호적선성관계，평균가양회수솔위99.4%；RSD소우2.0%。결론본방법쾌속、가고、간편，중복성호，전속성강，가위이진합제적질량표준제공삼고。
Objective To establish a method for the determination of hesperidine inErchen mixture. Methods HPLC method was used with Fortis C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-acetic acid-water(22∶4∶74). The flow rate was 1.0 ml/min,the column temperature was 30℃, and the UV detector was set at 283 nm.Results The linear ranges of hesperidine was 2.86-57.20μg(r=0.999 8). The average recovery of Aloin was 99.4%.Conclusion The method is rapid, reliable, simple, repeatable and with high specificity. It can be an important supplement for Erchen mixture elevated standard.