国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
10期
905-908
,共4页
钩藤总碱%缓释滴丸%大鼠%长期毒性
鉤籐總堿%緩釋滴汍%大鼠%長期毒性
구등총감%완석적환%대서%장기독성
Rhynchophylla total alkaloids%Sustained-release dropping pills%Rat%Long-term toxicity
目的：探讨钩藤总碱（RTA）缓释滴丸的长期毒性。方法将80只Wister大鼠按体质量随机分为空白对照组、RTA缓释滴丸高、中、低剂量组，每组20只。每天观察大鼠的一般状态、每2周记录一次体质量、日食量。连续灌胃12周后和停药2周后分别检测大鼠白细胞（WBC）、红细胞（RBC）、血红蛋白（Hb）、血小板（PLT）、淋巴细胞（LYM）、中性粒细胞（NEU）等血液学指标及谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、尿素氮（BUN）、肌酐（Cr）等生化指标，计算心、脑、肝、肾脏体比值，观察心、脑、肝、肾组织病理学改变。结果连续灌胃12周后， RTA 缓释滴丸高剂量组大鼠饮食减少，体质量增长缓慢；各组大鼠 WBC、RBC、Hb、PLT、LYM、NEU无明显变化；RTA缓释滴丸高剂量组大鼠的ALT、AST和ALP值升高[分别为(77.5±11.9)U/L、(210.4±21.7)U/L、(220.6±19.8)U/L]，与空白对照组[分别为(55.2±12.1)U/L、(180.4±21.3)U/L、(190.3±22.6)U/L]比较，差异有统计学意义（P＜0.05）。RTA 缓释滴丸高剂量组大鼠肝脏的脏体比值(3.86±0.29)较空白对照组(3.52±0.25)升高（P＜0.05），肝脏部分出现变性、灶状坏死。结论 RTA缓释滴丸高剂量长期给药可产生肝毒性。
目的：探討鉤籐總堿（RTA）緩釋滴汍的長期毒性。方法將80隻Wister大鼠按體質量隨機分為空白對照組、RTA緩釋滴汍高、中、低劑量組，每組20隻。每天觀察大鼠的一般狀態、每2週記錄一次體質量、日食量。連續灌胃12週後和停藥2週後分彆檢測大鼠白細胞（WBC）、紅細胞（RBC）、血紅蛋白（Hb）、血小闆（PLT）、淋巴細胞（LYM）、中性粒細胞（NEU）等血液學指標及穀丙轉氨酶（ALT）、穀草轉氨酶（AST）、堿性燐痠酶（ALP）、尿素氮（BUN）、肌酐（Cr）等生化指標，計算心、腦、肝、腎髒體比值，觀察心、腦、肝、腎組織病理學改變。結果連續灌胃12週後， RTA 緩釋滴汍高劑量組大鼠飲食減少，體質量增長緩慢；各組大鼠 WBC、RBC、Hb、PLT、LYM、NEU無明顯變化；RTA緩釋滴汍高劑量組大鼠的ALT、AST和ALP值升高[分彆為(77.5±11.9)U/L、(210.4±21.7)U/L、(220.6±19.8)U/L]，與空白對照組[分彆為(55.2±12.1)U/L、(180.4±21.3)U/L、(190.3±22.6)U/L]比較，差異有統計學意義（P＜0.05）。RTA 緩釋滴汍高劑量組大鼠肝髒的髒體比值(3.86±0.29)較空白對照組(3.52±0.25)升高（P＜0.05），肝髒部分齣現變性、竈狀壞死。結論 RTA緩釋滴汍高劑量長期給藥可產生肝毒性。
목적：탐토구등총감（RTA）완석적환적장기독성。방법장80지Wister대서안체질량수궤분위공백대조조、RTA완석적환고、중、저제량조，매조20지。매천관찰대서적일반상태、매2주기록일차체질량、일식량。련속관위12주후화정약2주후분별검측대서백세포（WBC）、홍세포（RBC）、혈홍단백（Hb）、혈소판（PLT）、림파세포（LYM）、중성립세포（NEU）등혈액학지표급곡병전안매（ALT）、곡초전안매（AST）、감성린산매（ALP）、뇨소담（BUN）、기항（Cr）등생화지표，계산심、뇌、간、신장체비치，관찰심、뇌、간、신조직병이학개변。결과련속관위12주후， RTA 완석적환고제량조대서음식감소，체질량증장완만；각조대서 WBC、RBC、Hb、PLT、LYM、NEU무명현변화；RTA완석적환고제량조대서적ALT、AST화ALP치승고[분별위(77.5±11.9)U/L、(210.4±21.7)U/L、(220.6±19.8)U/L]，여공백대조조[분별위(55.2±12.1)U/L、(180.4±21.3)U/L、(190.3±22.6)U/L]비교，차이유통계학의의（P＜0.05）。RTA 완석적환고제량조대서간장적장체비치(3.86±0.29)교공백대조조(3.52±0.25)승고（P＜0.05），간장부분출현변성、조상배사。결론 RTA완석적환고제량장기급약가산생간독성。
Objective To observe the long-term toxicity of sustained-release dropping pills of Rhynchophylla total alkaloids in rats.Methods 80 Wistar rats were randomly divided into high dose group, middle dose group, low dose group and normal group. The rats were consecutively drenched for 12 weeks. The general status of the animals was observed daily in medication duration and body weight, daily appetite, quantity were recorded every 2 weeks. All animals were sacrificed on drenched 12 weeks and 2 weeks after discontinuation, then the content of WBC, RBC, Hb, PLT, LYM, NEU and ALT, AST, ALP, BUN, Cr were detected. Ratio of viscera was measured and the major organs were examined by the pathology.Results Continuous intragastric administration for 12 weeks after high dose group rats, poor diet, weight growth was slow;no significant changes of Hematology was found;The ALT, AST and ALP of high dose group rats increased[respectively(77.5±11.9)U/L,(210.4±21.7)U/L,(220.6±19.8)U/L], compared with the blank control group[respectively(55.2±12.1)U/L,(180.4±21.3)U/L,(190.3±22.6)U/L], the difference was statistically significant(P<0.05). The ratio of liver body in high dose group(3.86±0.29)was higher than the control group(3.52±0.25), the difference was statistically significant(P<0.05). And liver degeneration, focal necrosis was found.Conclusion The main chronic toxic damage is liver damage caused by sustained-release dropping pills of Rhynchophylla total alkaloids of long-term large delivery.
