国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
10期
902-904
,共3页
王发善%李松%李铁男%褚弘斌%李杨%刘海洋%张宁
王髮善%李鬆%李鐵男%褚弘斌%李楊%劉海洋%張寧
왕발선%리송%리철남%저홍빈%리양%류해양%장저
五加生化胶囊提取物%MCF-7细胞%噻唑蓝比色法%植物雌激素样作用%有效作用部位
五加生化膠囊提取物%MCF-7細胞%噻唑藍比色法%植物雌激素樣作用%有效作用部位
오가생화효낭제취물%MCF-7세포%새서람비색법%식물자격소양작용%유효작용부위
Wujia-Shenghua capsule extract%MCF-7 cell%MTT test%Plant estrogen-like effects%Effective medication site
目的:观察五加生化胶囊的类雌激素样作用及有效药用部位。方法用 D-101型大孔树脂柱将五加生化胶囊60%乙醇提取物分离为水洗脱部位、20%乙醇洗脱部位、40%乙醇洗脱部位、60%乙醇洗脱部位,并将各洗脱部位分别配制成10、1、0.1、0.01 mg/ml 4个浓度,作用于MCF-7细胞,进行噻唑蓝(MTT)法检测,计算增殖率(PR)。结果在0.1 mg/ ml浓度时,水、20%乙醇及60%乙醇洗脱部位对 MCF-7细胞增殖均无影响(PR 分别为98.10%、101.06%、106.04%),与空白对照组比较(PR为100%),差异无统计学意义(P>0.05);40%乙醇洗脱部位可促进 MCF-7细胞增殖(PR为108.22%),差异有统计学意义(P<0.05)。在1 mg/ml浓度时,20%、40%及60%乙醇洗脱部位(PR分别为111.72%、122.48%、115.35%)均可明显促进MCF-7细胞增殖,差异有统计学意义(P<0.01)。结论五加生化胶囊40%乙醇洗脱部位植物雌激素活性最强。
目的:觀察五加生化膠囊的類雌激素樣作用及有效藥用部位。方法用 D-101型大孔樹脂柱將五加生化膠囊60%乙醇提取物分離為水洗脫部位、20%乙醇洗脫部位、40%乙醇洗脫部位、60%乙醇洗脫部位,併將各洗脫部位分彆配製成10、1、0.1、0.01 mg/ml 4箇濃度,作用于MCF-7細胞,進行噻唑藍(MTT)法檢測,計算增殖率(PR)。結果在0.1 mg/ ml濃度時,水、20%乙醇及60%乙醇洗脫部位對 MCF-7細胞增殖均無影響(PR 分彆為98.10%、101.06%、106.04%),與空白對照組比較(PR為100%),差異無統計學意義(P>0.05);40%乙醇洗脫部位可促進 MCF-7細胞增殖(PR為108.22%),差異有統計學意義(P<0.05)。在1 mg/ml濃度時,20%、40%及60%乙醇洗脫部位(PR分彆為111.72%、122.48%、115.35%)均可明顯促進MCF-7細胞增殖,差異有統計學意義(P<0.01)。結論五加生化膠囊40%乙醇洗脫部位植物雌激素活性最彊。
목적:관찰오가생화효낭적류자격소양작용급유효약용부위。방법용 D-101형대공수지주장오가생화효낭60%을순제취물분리위수세탈부위、20%을순세탈부위、40%을순세탈부위、60%을순세탈부위,병장각세탈부위분별배제성10、1、0.1、0.01 mg/ml 4개농도,작용우MCF-7세포,진행새서람(MTT)법검측,계산증식솔(PR)。결과재0.1 mg/ ml농도시,수、20%을순급60%을순세탈부위대 MCF-7세포증식균무영향(PR 분별위98.10%、101.06%、106.04%),여공백대조조비교(PR위100%),차이무통계학의의(P>0.05);40%을순세탈부위가촉진 MCF-7세포증식(PR위108.22%),차이유통계학의의(P<0.05)。재1 mg/ml농도시,20%、40%급60%을순세탈부위(PR분별위111.72%、122.48%、115.35%)균가명현촉진MCF-7세포증식,차이유통계학의의(P<0.01)。결론오가생화효낭40%을순세탈부위식물자격소활성최강。
ObjectiveTo observe the effect of estrogenic-like effects ofWujia-Shenghua capsule and effective medication site.MethodsThrough D-101 macroporous resin column methods,Wujia-Shenghua capsule with 60% ethanol extraction was separated for water elution part, 20% ethanol elution part, 40% ethanol elution part, 60% ethanol elution part. Then each elution part was respectively mixed into concentration of 10mg/ml,1mg/ml, 0.1mg/ml, 0.01 mg/ml and acted on the MCF-7 cell to have, MTT test and rate of PR calculation.Results Compared with the blank control group(100%), when the concentration was 0.1 mg/ml, water elution part, 20% ethanol elution part and 60% ethanol elution part(98.10%, 101.06%, 106.04%)had no effect on the proliferation of MCF-7 cells, there was not statistically significant(P>0.05), while the 40% ethanol elution part(108.22%)can promote the proliferation of MCF-7 cells,there was a statistically significant(P<0.05). When the concentration was 1 mg/ml, 20%, 40% and 60% ethanol elution part(111.72%, 122.48%, 115.35%)can distinctly promote the proliferation of MCF-7 cells, there was a significant difference between four groups(P<0.01).ConclusionThe 40% ethanol elution part ofWujia-shenghua capsule has the strongest estrogen activity on plant.