CAJ | 학술논문

目的：探讨三七花总皂苷清除活性氧自由基及对抗脂质过氧化作用的影响。方法从三七花盘中提取三七花总皂苷，D-101大孔吸附树脂分离纯化皂苷；采用分光光度法测定其对羟基自由基、DPPH 自由基的作用。用硫代巴比妥分光光度法观察三七花总皂苷对 Fe2＋半胱氨酸诱发肝脂质过氧化作用的影响。结果三七花总皂苷对羟自由基、1,1-二苯基-2-三硝基苯肼（DPPH）自由基的半数清除率（IC50值）分别为0.035 mg/ml、0.094 mg/ml；对Fe2＋半胱氨酸诱发肝脂质过氧化的最大抑制率为89.31%，具有较强的抑制作用。结论三七花总皂苷具有清除活性氧自由基及抗脂质过氧化作用。
목적：탐토삼칠화총조감청제활성양자유기급대항지질과양화작용적영향。방법종삼칠화반중제취삼칠화총조감，D-101대공흡부수지분리순화조감；채용분광광도법측정기대간기자유기、DPPH 자유기적작용。용류대파비타분광광도법관찰삼칠화총조감대 Fe2＋반광안산유발간지질과양화작용적영향。결과삼칠화총조감대간자유기、1,1-이분기-2-삼초기분정（DPPH）자유기적반수청제솔（IC50치）분별위0.035 mg/ml、0.094 mg/ml；대Fe2＋반광안산유발간지질과양화적최대억제솔위89.31%，구유교강적억제작용。결론삼칠화총조감구유청제활성양자유기급항지질과양화작용。
Objective To investigate the antioxidation activity and resistance of lipid peroxidation of panax notoginseng flower total saponins.Methods The panax notoginseng flower buds were extracted with ethanol. The hydroxyl free radical(?OH), 1,1-diphenyl-2-picrythydrazyl radical(DPPH)clearing rate and resistance of lipid peroxidation of rat liver induced by Fe2+-cysteine were determined by spectrophotometry. Results Half clearance of hydroxyl free radical and DPPH. by panax notoginseng flower total saponin was 0.035 mg/ml and 0.094 mg/ml, the maximum inhibition rate of lipid peroxidation of rat liver induced by Fe2+-cysteine was 89.31%, therefore moderate concentration of extracts had a strong inhibitory effect on lipid peroxidation. Conclusions Panax notoginseng flower total saponins have antioxidant activity and resistance of lipid peroxidation.