CAJ | 학술논문

目的：探讨神经纤毛蛋白2（NRP2）在胃癌组织中的表达及其与微淋巴管新生及临床病理因素的相关性。方法回顾性分析河北联合大学附属医院和唐山市人民医院2011年10月至2013年10月手术切除胃癌组织标本（每例患者分别取癌组织、癌旁组织及相对正常区组织）共65例资料，采用免疫组织化学法检测NRP2表达情况，选取D2-40单克隆抗体标记淋巴管，计数微淋巴管密度（ MLD），观测淋巴管形态。结果癌组织、癌旁组织及相对正常区组织各65例，其中胃癌组织NRP2阳性表达率高于癌旁组织和正常组织[61.5％（40／65）比49.2％（32／65）和4.6％（3／65），均P＜0.05]；胃癌组织和正常组织MLD值分别为（12±5）、（23±6）个／mm2，均低于癌旁组织[（44±13）个／mm2，均P＜0.05]，各组淋巴管形态也各不相同；同时癌旁NRP2的表达情况及MLD与肿瘤大小、细胞分化程度、组织浸润深度、淋巴管浸润、淋巴结转移及肿瘤TNM分期均相关（均P＜0.05）。NRP2的表达强度与MLD呈正相关（r＝0.517，P＝0.000）。结论 NRP2可能参与调节胃癌微淋巴管新生和淋巴道转移，在胃癌肿瘤发生、病期进展中具有关键作用，推断其可作为胃癌预后判断的相关指标。
목적：탐토신경섬모단백2（NRP2）재위암조직중적표체급기여미림파관신생급림상병리인소적상관성。방법회고성분석하북연합대학부속의원화당산시인민의원2011년10월지2013년10월수술절제위암조직표본（매례환자분별취암조직、암방조직급상대정상구조직）공65례자료，채용면역조직화학법검측NRP2표체정황，선취D2-40단극륭항체표기림파관，계수미림파관밀도（ MLD），관측림파관형태。결과암조직、암방조직급상대정상구조직각65례，기중위암조직NRP2양성표체솔고우암방조직화정상조직[61.5％（40／65）비49.2％（32／65）화4.6％（3／65），균P＜0.05]；위암조직화정상조직MLD치분별위（12±5）、（23±6）개／mm2，균저우암방조직[（44±13）개／mm2，균P＜0.05]，각조림파관형태야각불상동；동시암방NRP2적표체정황급MLD여종류대소、세포분화정도、조직침윤심도、림파관침윤、림파결전이급종류TNM분기균상관（균P＜0.05）。NRP2적표체강도여MLD정정상관（r＝0.517，P＝0.000）。결론 NRP2가능삼여조절위암미림파관신생화림파도전이，재위암종류발생、병기진전중구유관건작용，추단기가작위위암예후판단적상관지표。
Objective To explore the correlation between the expression of neuropilin-2 ( NRP2 ) and tumor-associated lymphangiogenesis and clinicopathological parameters in gastric carcinoma ( GC ).Methods All GC, margin and normal tissues of 65 cases through surgical resection were retrospectively collected at our hospital and Tangshan People′s Hospital from October 2011 to October 2013.The expressions of NRP2 in GC, tumor margin and normal gastric tissues were analyzed by immunohistochemistry.Lymphangiogenesis was marked with D 2-40 monoclonal antibody and microlymphatic density ( MLD) counted.And the morphology of lymphatic vessels was observed.Results A positive expression of NRP2 was found in GC ( n=40, 61.5%) and it was significantly higher than that in tumor margin (n=32, 49.2%) and normal tissues (n=3, 4.6%) (P<0.05).But the counts of MLD in both GC (12 ±5)/mm2 and normal region (23 ±6)/mm2 were significantly lower than that of tumor margin region (44 ±13)/mm2(P<0.05).In addition, the morphologies of lymphatic vessels were different among 3 groups.Also NRP2 expression and MLD count were correlated with differentiation degree , infiltrative degree, lymphatic vessel invasion, lymph node metastasis and TNM staging of GC (all P<0.05).The expression intensity of NRP2 in tumor margin tissues was positively correlated with MLD ( r=0.517, P=0.000).Conclusions The expression of NRP2 in GC may modulate lymphangiogenesis and lymphatic metastasis.And it may play a key role in tumorigenesis , progression and prognosis predicating in GC.