CAJ | 학술논문

目的：比较两种破碎方法提取的蛋白质样品在双向电泳（2-DE）中的分离效果。方法用超声破碎和玻璃珠振荡破碎方法分别制备革兰阴性菌、革兰阳性菌、动物组织的蛋白抽提样品，通过2-DE比较两种制样方法的实际效果。结果获得了革兰阴性菌、革兰阳性菌、动物组织在两种制样条件下的2-DE图。结论通过对比不同样品的电泳结果发现，玻璃珠振荡破碎法制样背景更低，更有利于后续的图像分析。
목적：비교량충파쇄방법제취적단백질양품재쌍향전영（2-DE）중적분리효과。방법용초성파쇄화파리주진탕파쇄방법분별제비혁란음성균、혁란양성균、동물조직적단백추제양품，통과2-DE비교량충제양방법적실제효과。결과획득료혁란음성균、혁란양성균、동물조직재량충제양조건하적2-DE도。결론통과대비불동양품적전영결과발현，파리주진탕파쇄법제양배경경저，경유리우후속적도상분석。
Objective To compare the separation effects of protein samples extracted by two different methods with two -dimensional gel electrophoresis (2-DE) .Methods Ultrasonic disruption and glass-beads grounding were used to prepare protein samples of Gram-negative bacteria , Gram-positive bacteria and animal tissues .The actual results of the two sample preparation methods were compared by 2-DE.Results The 2-DE maps of samples extracted by the two methods were obtained.Conclusion The 2-DE maps of glass-beads grounding samples are better than those of ultrasonic disruption thanks to their lower backgrounds , which are beneficial for further image analyses .