中国性科学
中國性科學
중국성과학
THE CHINESE JOURNAL OF HUMAN SEXUALITY
2014年
9期
43-45
,共3页
陈昊宾%张敏%罗贤勇%陈春惠
陳昊賓%張敏%囉賢勇%陳春惠
진호빈%장민%라현용%진춘혜
子宫颈肿瘤%子宫颈上皮内病变%病理学,临床%免疫组织化学%P16%P53%P63%Ki-67
子宮頸腫瘤%子宮頸上皮內病變%病理學,臨床%免疫組織化學%P16%P53%P63%Ki-67
자궁경종류%자궁경상피내병변%병이학,림상%면역조직화학%P16%P53%P63%Ki-67
Cervical cancer%Cervical intraepithelial lesions%Pathology%Clinical%Immunohisto-chemistry%P1 6%P53%P63%Ki-67
目的：探讨子宫颈上皮内病变（CIN ）组织中免疫组织化学的应用及其临床病理学意义。方法：选择256例子宫颈活检组织标本，采用免疫组织化学技术检测P16、P53、P63和Ki-67表达，其中30例正常子宫颈组织为对照组，CIN170例、CIN280例、CIN376例。结果：正常鳞状上皮P16、P53、P63和Ki-67免疫组化染色均为阴性或弱阳性，而在 CIN 中各级病变均有较高表达，且随 CIN 级别升高而表达增强，各组间有差别并与 CIN 分级呈正相关（P＜0.01）。同时P16、P63二者阳性表达均见分层现象，在 CIN1中细胞的阳性表达大多局限于宫颈鳞状上皮的下1/3，在 CIN2中阳性细胞达皮下2/3，而 CIN3中则在上皮下2/3直至全层弥漫阳性。P53和Ki-67的阳性表达从 CIN1到 CIN3逐渐增加，多组之间比较及 CIN1与CIN2两组之间比较差别有统计学意义（P＜0.01），但CIN2与 CIN3两组之间的比较差异无统计学意义（P＞0.05）。结论：在子宫颈病变中检测P16、P53、P63和Ki-67蛋白的表达，有助于子宫颈癌前病变的筛查、早期诊断和治疗。
目的：探討子宮頸上皮內病變（CIN ）組織中免疫組織化學的應用及其臨床病理學意義。方法：選擇256例子宮頸活檢組織標本，採用免疫組織化學技術檢測P16、P53、P63和Ki-67錶達，其中30例正常子宮頸組織為對照組，CIN170例、CIN280例、CIN376例。結果：正常鱗狀上皮P16、P53、P63和Ki-67免疫組化染色均為陰性或弱暘性，而在 CIN 中各級病變均有較高錶達，且隨 CIN 級彆升高而錶達增彊，各組間有差彆併與 CIN 分級呈正相關（P＜0.01）。同時P16、P63二者暘性錶達均見分層現象，在 CIN1中細胞的暘性錶達大多跼限于宮頸鱗狀上皮的下1/3，在 CIN2中暘性細胞達皮下2/3，而 CIN3中則在上皮下2/3直至全層瀰漫暘性。P53和Ki-67的暘性錶達從 CIN1到 CIN3逐漸增加，多組之間比較及 CIN1與CIN2兩組之間比較差彆有統計學意義（P＜0.01），但CIN2與 CIN3兩組之間的比較差異無統計學意義（P＞0.05）。結論：在子宮頸病變中檢測P16、P53、P63和Ki-67蛋白的錶達，有助于子宮頸癌前病變的篩查、早期診斷和治療。
목적：탐토자궁경상피내병변（CIN ）조직중면역조직화학적응용급기림상병이학의의。방법：선택256례자궁경활검조직표본，채용면역조직화학기술검측P16、P53、P63화Ki-67표체，기중30례정상자궁경조직위대조조，CIN170례、CIN280례、CIN376례。결과：정상린상상피P16、P53、P63화Ki-67면역조화염색균위음성혹약양성，이재 CIN 중각급병변균유교고표체，차수 CIN 급별승고이표체증강，각조간유차별병여 CIN 분급정정상관（P＜0.01）。동시P16、P63이자양성표체균견분층현상，재 CIN1중세포적양성표체대다국한우궁경린상상피적하1/3，재 CIN2중양성세포체피하2/3，이 CIN3중칙재상피하2/3직지전층미만양성。P53화Ki-67적양성표체종 CIN1도 CIN3축점증가，다조지간비교급 CIN1여CIN2량조지간비교차별유통계학의의（P＜0.01），단CIN2여 CIN3량조지간적비교차이무통계학의의（P＞0.05）。결론：재자궁경병변중검측P16、P53、P63화Ki-67단백적표체，유조우자궁경암전병변적사사、조기진단화치료。
Objectives:To investigate the application and clinical significance of immunohistochemical technique on cervical intraepithelial neoplasia.Methods:256 cases of cervical biopsy specimens were selected.The expression of p1 6 ,p53,p63and Ki-67 protein was detected by immunohistochemical technique.There were 30 ca-ses of normal cervical epitheliums in control group,70 cases in CIN1 group,80 cases in CIN2 group and 76 cases in CIN3 group.Results:Immunohistochemical staining of P1 6,P53,P63 and Ki-67 in normal squamous epithelium were negative or weakly positive and P1 6,P53,P63 and Ki-67 in CIN group had higher levels of expression.The level of expression went higher along with the CIN level.Expression between groups was statistically significant (P<0.01 )and positively correlated.Meanwhile,expression of P1 6 and P63 was in stratification.Expression of P53 and Ki-67 gradually increased from CIN1 group to CIN3 group.The differences between multiple groups and between CIN1 group and CIN2 group were statistically significant (P<0.01 ),but the difference between CIN2 group and CIN3 group was not statistically significant (P>0.05).Conclusions:The detection of expression of P1 6,P53,P63 and Ki-67 proteins in cervical lesions is helpful for the screening,early diagnosis and treatment of precancerous le-sions of cervix.
