CAJ | 학술논문

前期研究发现，楸树叶粗提物的乙酸乙酯萃取相具有较强的抗氧化活性。为了进一步明确其抗氧化活性成分，以硅胶柱色谱结合清除 DPPH自由基法，寻找抗氧化能力较强的组分，并利用半制备液相色谱对该组分进行分离、纯化，以清除 DPPH自由基、还原力和清除 ABTS自由基能力作为考察指标，根据理化性质及光谱数据鉴定化合物。结果从楸树叶粗提物的乙酸乙酯萃取相中分离得到了2个化合物，分别为木犀草素(1)和芹菜素(2)。化合物1的抗氧化活性强于BHT和化合物2；化合物1清除DPPH自由基、还原力及清除ABTS自由基的IC50值分别为23.89，29.77和43.47μg mL -1；化合物1的抗氧化活性强于化合物2，主要因其在黄酮类化合物母核的 B环上具有邻二酚羟基结构。木犀草素和芹菜素2个化合物首次从楸树叶中分离得到。
전기연구발현，추수협조제물적을산을지췌취상구유교강적항양화활성。위료진일보명학기항양화활성성분，이규효주색보결합청제 DPPH자유기법，심조항양화능력교강적조분，병이용반제비액상색보대해조분진행분리、순화，이청제 DPPH자유기、환원력화청제 ABTS자유기능력작위고찰지표，근거이화성질급광보수거감정화합물。결과종추수협조제물적을산을지췌취상중분리득도료2개화합물，분별위목서초소(1)화근채소(2)。화합물1적항양화활성강우BHT화화합물2；화합물1청제DPPH자유기、환원력급청제ABTS자유기적IC50치분별위23.89，29.77화43.47μg mL -1；화합물1적항양화활성강우화합물2，주요인기재황동류화합물모핵적 B배상구유린이분간기결구。목서초소화근채소2개화합물수차종추수협중분리득도。
Our previous study found that ethyl acetate extract phase of Catalpa bungei leaves had strong antioxidant activity. In order to further clarify its antioxidant activity,we have searched the components had strong antioxidant ability though silica gel column chromatography combined with DPPH free radical scavenging methods. The antioxidant components were isolated and purified by semi preparative liquid chromatography. With DPPH radical scavenging activity, reducing capacity and ABTS radical scavenging activity ability as investigation index to find the antioxidant components and the physicochemical properties and spectral data were to identify compounds. The results showed that the ethyl acetate extract phase of C. bungei leaves had two compounds: luteolin ( 1 ) and apigenin ( 2 ) . The antioxidant activity of compound 1 was stronger than BHT and compound 2. DPPH radical scavenging activity,reducing power and ABTS radical scavenging activity IC50 values of compound 1 were 23. 89,29. 77 and 43. 47 μg·mL -1 respectively. The antioxidant activity of compound 1 was stronger than compound 2 mainly because of its adjacent structure of two phenolic hydroxyl groups in the flavone compound nucleus of B ring. Luteolin and apigenin isolated from C. bungei leaves for the first time.