林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2014年
9期
51-58
,共8页
曾宪君%李丹%胡彦鹏%黄秦军%苏晓华
曾憲君%李丹%鬍彥鵬%黃秦軍%囌曉華
증헌군%리단%호언붕%황진군%소효화
欧洲黑杨%优质核心种质%抽样方法%遗传多样性
歐洲黑楊%優質覈心種質%抽樣方法%遺傳多樣性
구주흑양%우질핵심충질%추양방법%유전다양성
Populus nigra%high-quality core collection%sampling method%genetic diversity
对159份欧洲黑杨种质资源的生长、育种值、材性、养分利用率和水分利用率等13个性状进行分析,采用13种表型选优的抽样方法构建欧洲黑杨优质核心种质库。表型值评价表明:当抽样群体比例达到21.4%以上即能满足构建核心种质要求,而只有抽样群体比例达到30.2%以上 SSR 分子遗传多样性才能满足构建核心种质要求。最终确定的欧洲黑杨优质核心种质库包含48份种质资源,占原群体的30.2%;与原群体相比,均值和方差不存在显著差异,极差符合率和变异系数变化率分别为97.07%和89.72%;优质核心种质库的有效等位基因数、Nei’s 遗传多样性系数和 Shannon’s 信息指数与原群体之间没有显著差异,保持了原群体的遗传多样性。
對159份歐洲黑楊種質資源的生長、育種值、材性、養分利用率和水分利用率等13箇性狀進行分析,採用13種錶型選優的抽樣方法構建歐洲黑楊優質覈心種質庫。錶型值評價錶明:噹抽樣群體比例達到21.4%以上即能滿足構建覈心種質要求,而隻有抽樣群體比例達到30.2%以上 SSR 分子遺傳多樣性纔能滿足構建覈心種質要求。最終確定的歐洲黑楊優質覈心種質庫包含48份種質資源,佔原群體的30.2%;與原群體相比,均值和方差不存在顯著差異,極差符閤率和變異繫數變化率分彆為97.07%和89.72%;優質覈心種質庫的有效等位基因數、Nei’s 遺傳多樣性繫數和 Shannon’s 信息指數與原群體之間沒有顯著差異,保持瞭原群體的遺傳多樣性。
대159빈구주흑양충질자원적생장、육충치、재성、양분이용솔화수분이용솔등13개성상진행분석,채용13충표형선우적추양방법구건구주흑양우질핵심충질고。표형치평개표명:당추양군체비례체도21.4%이상즉능만족구건핵심충질요구,이지유추양군체비례체도30.2%이상 SSR 분자유전다양성재능만족구건핵심충질요구。최종학정적구주흑양우질핵심충질고포함48빈충질자원,점원군체적30.2%;여원군체상비,균치화방차불존재현저차이,겁차부합솔화변이계수변화솔분별위97.07%화89.72%;우질핵심충질고적유효등위기인수、Nei’s 유전다양성계수화 Shannon’s 신식지수여원군체지간몰유현저차이,보지료원군체적유전다양성。
In this study,we analyzed 13 traits of 159 Populus nigra germplasm such as growth,breeding value,material properties,efficiency of water use efficiency and efficiency of nutrient utilization. A high-quality core collection of P. nigra was constructed by utilizing 13 kinds of sampling methods of phenotypic selection. Phenotypic value evaluation showed that the sampling rate reached to more than 21. 4% can meet the requirement of building core collection. However,the proportion of the sample population over 30. 2% was required for building the core collection in terms of SSR molecular genetic diversity. Ultimately,the determined high-quality core collection of P. nigra contained 48 germplasm resources,accounting for 30. 2% of the original germplasm. Compared with the original germplasm,there were no differences in the aspects of the mean and the variance,and the coincidence rate of range and the changeable rate of coefficient of variation were 97. 07%and 89. 72%. The high-quality core collection was no significantly different from the original group in the aspects of the effective number of alleles,Nei’s genetic diversity coefficient and Shannon’s information index,suggesting that the high-quality core collection should maintain the genetic diversity of the original group.