CAJ | 학술논문

通过对油茶成花过程的转录组测序及其成花相关基因的表达分析，结果表明：转录组测序总共获得28448847个 reads，5742023480 bp 数据量，GC 含量为46.52%；拼接成大于200 bp 以上的 Unigenes 有94476条，N50长度为806 bp，其中1 kbp以上的Unigenes 共12643条，占Unigene总数的13.38%； Unigenes 在各数据库中功能注释数目，在 COG中有9095条，在 GO中有27201条，在 KEGG中有6431条，在 Swissprot中有24534条，在TrEMBL中有36393条，在Nr中有36400条，在Nt中有30858条；茎尖中FLC，FCA和FT基因表达量较 AP1，AP2和 PI 基因低，FT基因( ID：Unigene60063)是油茶成花的关键基因，PI 基因( ID：Unigene56059)与雄蕊发育关系紧密。
통과대유다성화과정적전록조측서급기성화상관기인적표체분석，결과표명：전록조측서총공획득28448847개 reads，5742023480 bp 수거량，GC 함량위46.52%；병접성대우200 bp 이상적 Unigenes 유94476조，N50장도위806 bp，기중1 kbp이상적Unigenes 공12643조，점Unigene총수적13.38%； Unigenes 재각수거고중공능주석수목，재 COG중유9095조，재 GO중유27201조，재 KEGG중유6431조，재 Swissprot중유24534조，재TrEMBL중유36393조，재Nr중유36400조，재Nt중유30858조；경첨중FLC，FCA화FT기인표체량교 AP1，AP2화 PI 기인저，FT기인( ID：Unigene60063)시유다성화적관건기인，PI 기인( ID：Unigene56059)여웅예발육관계긴밀。
In this study,total RNA of stem apex and bud in Camellia oleifera was sequenced and its flowering relation genes were analysis. The results showed that transcriptome sequencing revealed a total of 28 448 847 reads, 5 742 023 480 bp data,and 46. 52% GC content in whole genome. The Unigenes numbers with more than 200 bp were 94 476,and the Unigenes were very similar with grape’s. The N50 length was 806 bp,and 1 kbp or longer Unigenes were 12 643,accounting for 13. 38% of total Unigenes database. The annotation number of Unigenes function in each database was :9 095 Unigenes in COG,27 201 Unigenes in GO,6 431 Unigenes in KEGG,24 534 Unigenes in Swissprot,36 393 Unigenes in TrEMBL,36 400 Unigenes in Nr,and 30 858 Unigenes in Nt. The expression profile showed that FLC,FCA,FT expression were lower than AP1,AP2,PI expression in the stem apex. The FT gene ( ID:Unigene60063) may be a key gene for Camellia flower,and PI genes (ID:Unigene56059) are closely related with the stamen development.