CAJ | 학술논문

以降血糖药物格列美脲为模板分子，a-甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，偶氮二异丁腈（AIBN）为引发剂，采用本体聚合法制备了格列美脲分子印迹聚合物（MIP），用于血浆样品前处理，建立了对加标兔血浆中格列美脲含量的固相萃取检测方法，通过高效液相色谱法测定，分子印迹固相萃取柱的回收率可达80%以上，有效地减少了基体中蛋白质等杂质对目标物检测的干扰，适用于生物样品中模板分子的富集和纯化。
이강혈당약물격렬미뇨위모판분자，a-갑기병희산（MAA）위공능단체，을이순이갑기병희산지（EDMA）위교련제，우담이이정정（AIBN）위인발제，채용본체취합법제비료격렬미뇨분자인적취합물（MIP），용우혈장양품전처리，건립료대가표토혈장중격렬미뇨함량적고상췌취검측방법，통과고효액상색보법측정，분자인적고상췌취주적회수솔가체80%이상，유효지감소료기체중단백질등잡질대목표물검측적간우，괄용우생물양품중모판분자적부집화순화。
Molecular imprinting polymer(MIP)for glimepiride was synthesized via a bulk polmerization route by using antihyperglycemic glimepiride as the template molecule,α-methacrylic acid(MAA)as the functional monomer,ethylene glycoldimethacrylate(EDMA)as the crosslinker,2,2’-azobis(2-methyl-propionitrile)(AIBN))as the initiator. As-prepared MIP was utilized as the sorbent of solid-phase extrac-tion for rabbit plasma sample pretreatment and a solid phase extraction method for the recovery of glimepiride in spiked rabbit plasma was established combining with high-performance liquid chromatogra-phy. Results show that the recovery rates of MIP extraction column reach above 80% ,effectively reducing the interferences from the matrix proteins and other impurities to the analysis of target molecule glimepiride.