CAJ | 학술논문

目的：探讨剑麻皂素对大鼠局灶性脑缺血再灌注损伤的保护作用及其机制。方法采用大脑中动脉闭塞法制备局灶性脑缺血再灌注损伤大鼠模型，模型建立成功后，选取大鼠60只，按随机数字表法分为模型组、三七通舒组，剑麻皂素高、中、低剂量组，每组12只。模型组灌服蒸馏水，三七通舒组灌服三七通舒胶囊，剑麻皂素高、中、低剂量组分别按450、225、113 mg/（kg? d）灌服剑麻皂素。对比各组神经行为学评分、脑梗死体积、脑组织含水量及血清超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、乳酸（LA）水平。结果剑麻皂素高、中剂量组大鼠神经行为学评分明显低于模型组（P ＜0．05），绝对脑梗死体积和相对脑梗死体积明显小于模型组（P ＜0．05），剑麻皂素高剂量组脑含水量明显低于模型组（P ＜0．05）。剑麻皂素高剂量组大鼠血清 SOD、GSH-Px 水平明显高于模型组，MDA、LA 水平明显低于模型组（P ＜0．05）；剑麻皂素中剂量组大鼠血清 SOD 水平明显高于模型组（P ＜0．05），MDA 水平低于模型组（P ＜0．05）。结论剑麻皂素对大鼠局灶性脑缺血再灌注损伤具有明显保护作用，其机制可能与清除自由基、减轻 LA 性酸中毒程度有关。
목적：탐토검마조소대대서국조성뇌결혈재관주손상적보호작용급기궤제。방법채용대뇌중동맥폐새법제비국조성뇌결혈재관주손상대서모형，모형건립성공후，선취대서60지，안수궤수자표법분위모형조、삼칠통서조，검마조소고、중、저제량조，매조12지。모형조관복증류수，삼칠통서조관복삼칠통서효낭，검마조소고、중、저제량조분별안450、225、113 mg/（kg? d）관복검마조소。대비각조신경행위학평분、뇌경사체적、뇌조직함수량급혈청초양화물기화매（SOD）、병이철（MDA）、곡광감태과양화물매（GSH-Px）、유산（LA）수평。결과검마조소고、중제량조대서신경행위학평분명현저우모형조（P ＜0．05），절대뇌경사체적화상대뇌경사체적명현소우모형조（P ＜0．05），검마조소고제량조뇌함수량명현저우모형조（P ＜0．05）。검마조소고제량조대서혈청 SOD、GSH-Px 수평명현고우모형조，MDA、LA 수평명현저우모형조（P ＜0．05）；검마조소중제량조대서혈청 SOD 수평명현고우모형조（P ＜0．05），MDA 수평저우모형조（P ＜0．05）。결론검마조소대대서국조성뇌결혈재관주손상구유명현보호작용，기궤제가능여청제자유기、감경 LA 성산중독정도유관。
Objective To explore the protective effects of tigogenine on rats with focal cerebral ischemia-reperfusion injury and its mechanism.Methods Copied the model of focal cerebral ischemia reperfusion in rats by middle cerebral artery occlusion(MCAO).Then 60 rats were randomly divided into model group ,Sanqitongshu group,high-,middle-and low-dose tigogenine groups,with 12 rats in each group.The model group was given distilled water by gavage,Sanqitongshu group was given Sanqitongshu capsule by gavage,high-,middle-and low-dose tigogenine groups were given tigogenine of 450 mg/(kg? d),225 mg/(kg? d)and 113 mg/(kg? d),respectively,by gavage.The neuroethology score,volume of cerebral infarction,cerebral water content and the serum superoxide dismutase (SOD),malonaldehyde(MDA),glutathione peroxidase (GSH-Px),lactic acid(LA) levels were compared among groups.Results The neuroethology score of high-and middle-dose tigogenine groups was significantly lower than that of model group(P <0.05),the absolute and relative volumes of ischemia of high-and middle-dose tigogenine groups were significantly smaller than those of model group(P <0.05),the cerebral water content of high-dose tigogenine group was significantly lower than that of model group(P <0.05).The serum SOD and GSH-Px levels of high-dose tigogenine group were significantly higher than those of model group while the MDA and LA levels were significantly lower than those of model group (P <0.05).The serum SOD level of middle-dose tigogenine group was significantly higher than that of model group (P <0.05),but the MDA level was lower than that of model group (P <0.05).Conclusion Tigogenine has the protective effect on rats with focal cerebral ischemia -reperfusion injury, which might be related with anti-free radical,anti-oxidation function and relieving lactic acidosis .