CAJ | 학술논문

目的：探讨前列腺素内过氧化物合成酶2（PTGS2）基因启动子区单核苷酸多态性与原发性肝癌预后的关系。方法采用聚合酶链反应-限制性片段长度多态性分析法分析 PTGS2基因启动子区-1195G ＞A和-765G ＞C的基因型，使用 Kaplan-Meier 法和 Cox 比例风险回归模型分析不同基因型肝癌患者的预后。结果Kaplan-Meier 生存分析显示，携带-1195AG 基因型及携带-1195AG ＋AA 基因型的患者无疾病生存期（DFS）较携带-1195GG基因型的患者明显缩短（P ＜0．05）。-765G ＞C 位点多态性与肝癌患者 DFS 无显著相关性（P ＞0．05）。多因素Cox 回归分析显示，术前甲胎蛋白水平≤400 ng/ml 是影响 DFS 的独立因素（P ＜0．05）；-1195G ＞A及-765G ＞C位点多态性不是影响肝癌预后的独立影响因素（P ＞0．05）。结论携带-1195AG 基因型及 A 等位基因的肝癌患者术后容易较早发生疾病进展，-1195G ＞A 及-765G ＞C 位点多态性不是肝癌预后的独立影响因素。
목적：탐토전렬선소내과양화물합성매2（PTGS2）기인계동자구단핵감산다태성여원발성간암예후적관계。방법채용취합매련반응-한제성편단장도다태성분석법분석 PTGS2기인계동자구-1195G ＞A화-765G ＞C적기인형，사용 Kaplan-Meier 법화 Cox 비례풍험회귀모형분석불동기인형간암환자적예후。결과Kaplan-Meier 생존분석현시，휴대-1195AG 기인형급휴대-1195AG ＋AA 기인형적환자무질병생존기（DFS）교휴대-1195GG기인형적환자명현축단（P ＜0．05）。-765G ＞C 위점다태성여간암환자 DFS 무현저상관성（P ＞0．05）。다인소Cox 회귀분석현시，술전갑태단백수평≤400 ng/ml 시영향 DFS 적독립인소（P ＜0．05）；-1195G ＞A급-765G ＞C위점다태성불시영향간암예후적독립영향인소（P ＞0．05）。결론휴대-1195AG 기인형급 A 등위기인적간암환자술후용역교조발생질병진전，-1195G ＞A 급-765G ＞C 위점다태성불시간암예후적독립영향인소。
Objective To explore the relationship between the single nucleotide polymorphism (SNP) of promoter region of prostaglandin endoperoxide synthase 2(PTGS2) gene and the prognosis of primary hepatocellular carcinoma (HCC).Methods The method of polymerase chain reaction -restriction fragment length ployorphism(PCR-RFLP) was used to analyze the genotype of PTGS2 gene promoter -1195G >A and -765G >C.Kaplan-Meier method and Cox′s proportional hazards regression model were used to analyze the prognosis of patients with primary hepatocellular carcinoma of different genotypes.Results Kaplan-Meier survival analysis showed that the disease-free survival(DFS) for patients with-1195AG genotype or -1195AG +AA genotype was significantly shorter than that with the -1195GG genotype(P <0.05). The polymorphism of -765G >C locus didn′t correlated with DFS in patients with HCC(P >0.05).Multivariate Cox regression analysis showed that alpha -fetoprotein level lower than or equivalent to 400 ng /ml before operation was the independent influence factor of DFS(P <0.05),while the -1195G >A and -765G >C polymorphisms were not the independent influence factors of HCC prognosis (P >0.05).Conclusion Disease progression could be easily observed in HCC patients with -1195AG genotype and A allele.And the -1195G >A and -765G >C polymorphisms aren′t the independent influence factors of HCC prognosis .