CAJ | 학술논문

目的：探讨高糖高脂条件下内皮细胞与系膜细胞相互作用对血小板活化因子（PAF）产生、系膜细胞血小板活化因子受体（PAF-R）基因表达的影响及阿托伐他汀的干预作用。方法内皮细胞与系膜细胞共培养和系膜细胞单独培养，分为对照组、甘露醇组、高糖＋高溶血卵磷脂组、阿托伐他汀干预组，采用实时荧光定量检测系膜细胞 PAF-R mRNA 表达、酶联免疫吸附法检测 PAF 含量。结果共培养组和单培养组在高糖高溶血卵磷脂条件下，PAF 和系膜细胞 PAF-R mRNA 表达均升高（P ＜0．05），单培养 PAF 均较共培养下降（P＜0．05）；阿托伐他汀可抑制高糖高溶血卵磷脂引起的 PAF 和系膜细胞 PAF-R mRNA 表达上调（P ＜0．05）。结论系膜细胞和内皮细胞在高糖高溶血卵磷脂培养下，存在相互作用，并促进 PAF 产生和系膜细胞 PAF-R基因表达。阿托伐他汀可影响高糖高脂条件下内皮细胞与系膜细胞之间的相互作用，减少 PAF 的产生及下调系膜细胞 PAF-R 基因表达。
목적：탐토고당고지조건하내피세포여계막세포상호작용대혈소판활화인자（PAF）산생、계막세포혈소판활화인자수체（PAF-R）기인표체적영향급아탁벌타정적간예작용。방법내피세포여계막세포공배양화계막세포단독배양，분위대조조、감로순조、고당＋고용혈란린지조、아탁벌타정간예조，채용실시형광정량검측계막세포 PAF-R mRNA 표체、매련면역흡부법검측 PAF 함량。결과공배양조화단배양조재고당고용혈란린지조건하，PAF 화계막세포 PAF-R mRNA 표체균승고（P ＜0．05），단배양 PAF 균교공배양하강（P＜0．05）；아탁벌타정가억제고당고용혈란린지인기적 PAF 화계막세포 PAF-R mRNA 표체상조（P ＜0．05）。결론계막세포화내피세포재고당고용혈란린지배양하，존재상호작용，병촉진 PAF 산생화계막세포 PAF-R기인표체。아탁벌타정가영향고당고지조건하내피세포여계막세포지간적상호작용，감소 PAF 적산생급하조계막세포 PAF-R 기인표체。
Objective To investigate the role of platelet activating factor (PAF) and the expression of platelet activating factor receptor (PAF-R) in mesangial cells affected by the interaction of endothelial cells and mesangial cells exposed to high glucose and lysophosphatidylcholine (LPC),and to explore the effect of atorvastatin intervention . Methods Endothelial cells and mesangial cells were co -cultured while mesangial cells were cultured alone .The model of intercellular interaction between endothelial cells and mesangial cells was established and divided into 4 groups:control group,mannitol group ,high glucose and LPC group,and atorvastatin group.The expression of PAF-R mRNA in mesangial cells was determined by quantitative real -time PCR.The content of PAF in mesangial cells was determined by enzyme-linked immunosorbent assay( ELISA).Results The content of PAF and the expression level of PAF-R in mesangial cells increased when exposed to high glucose and LPC in co-culture and single-culture groups(P <0.05).The content of PAF of the monolayer cell culture was lower than that of the co-culture(P <0.05).Atorvastatin could inhibit the up-regulation of PAF and PAF-R mRNA expression in mesangial cells induced by high glucose and LPC(P <0.05).Conclusion Exposed to high glucose and LPC,endothelial cells and mesangial cells can interact with each other to produce PAF.The expression of PAF-R in mesangial cells is promoted by high glucose and LPC.The intercellular interaction between endothelial cells and mesangial cells may be intervened by atorvastatin,atorvastatin can eliminate the excess of the PAF and down -regulate the expression of PAF-R in mesangial cells when exposed to high glucose and LPC .