浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2014年
18期
1535-1537,1576
,共4页
植物雌激素%三羟异黄酮%子宫%子宫内膜%安全性
植物雌激素%三羥異黃酮%子宮%子宮內膜%安全性
식물자격소%삼간이황동%자궁%자궁내막%안전성
Phytoestrogen%Genistein%Uterus%Endometrium%Safety
目的：探讨不同剂量的植物雌激素三羟异黄酮（GST）对子宫质量及内膜的影响。方法选取50只成熟雌性SD大鼠，随机分为5组：假手术组、去卵巢组、去卵巢+GST低剂量组（25mg/kg）、去卵巢+GST中剂量组（50mg/kg）、去卵巢+GST高剂量组（100mg/kg），后3组每天以不同剂量GST溶液灌胃，实验持续4周。结果实验结束时，各组大鼠体质量较实验前均有增加，差异均有统计学意义（均P＜0.05）,但组间比较差异无统计学意义（P＞0.05）；GST低、中、高剂量组子宫质量分别为（0.18±0.03）、（0.16±0.04）、（0.17±0.05）g；对应的子宫质量/体质量分别为0.64±0.05、0.53±0.08、0.58±0.03，3组间的差异无统计学意义（P＞0.05）；分别与去卵巢组比较[子宫质量（0.15±0.05）g、子宫质量/体质量0.48±0.08]，差异均无统计学意义（均P＞0.05）；但与假手术组比较[子宫质量（0.45±0.08）g、子宫质量/体质量3.45±0.25]，差异均有统计学意义（均P＜0.01）。显微镜下观察大鼠子宫内膜，去卵巢组子宫内膜腺体小而稀少，使用不同剂量GST的低、中及高剂量组子宫内膜仍与去卵巢组一样，分布不均匀，腺体内衬上皮由立方或扁平细胞组成，明显富于细胞。结论不同剂量GST均不会促进去势大鼠子宫质量增加及子宫内膜增生，在临床应用可能是安全的。
目的：探討不同劑量的植物雌激素三羥異黃酮（GST）對子宮質量及內膜的影響。方法選取50隻成熟雌性SD大鼠，隨機分為5組：假手術組、去卵巢組、去卵巢+GST低劑量組（25mg/kg）、去卵巢+GST中劑量組（50mg/kg）、去卵巢+GST高劑量組（100mg/kg），後3組每天以不同劑量GST溶液灌胃，實驗持續4週。結果實驗結束時，各組大鼠體質量較實驗前均有增加，差異均有統計學意義（均P＜0.05）,但組間比較差異無統計學意義（P＞0.05）；GST低、中、高劑量組子宮質量分彆為（0.18±0.03）、（0.16±0.04）、（0.17±0.05）g；對應的子宮質量/體質量分彆為0.64±0.05、0.53±0.08、0.58±0.03，3組間的差異無統計學意義（P＞0.05）；分彆與去卵巢組比較[子宮質量（0.15±0.05）g、子宮質量/體質量0.48±0.08]，差異均無統計學意義（均P＞0.05）；但與假手術組比較[子宮質量（0.45±0.08）g、子宮質量/體質量3.45±0.25]，差異均有統計學意義（均P＜0.01）。顯微鏡下觀察大鼠子宮內膜，去卵巢組子宮內膜腺體小而稀少，使用不同劑量GST的低、中及高劑量組子宮內膜仍與去卵巢組一樣，分佈不均勻，腺體內襯上皮由立方或扁平細胞組成，明顯富于細胞。結論不同劑量GST均不會促進去勢大鼠子宮質量增加及子宮內膜增生，在臨床應用可能是安全的。
목적：탐토불동제량적식물자격소삼간이황동（GST）대자궁질량급내막적영향。방법선취50지성숙자성SD대서，수궤분위5조：가수술조、거란소조、거란소+GST저제량조（25mg/kg）、거란소+GST중제량조（50mg/kg）、거란소+GST고제량조（100mg/kg），후3조매천이불동제량GST용액관위，실험지속4주。결과실험결속시，각조대서체질량교실험전균유증가，차이균유통계학의의（균P＜0.05）,단조간비교차이무통계학의의（P＞0.05）；GST저、중、고제량조자궁질량분별위（0.18±0.03）、（0.16±0.04）、（0.17±0.05）g；대응적자궁질량/체질량분별위0.64±0.05、0.53±0.08、0.58±0.03，3조간적차이무통계학의의（P＞0.05）；분별여거란소조비교[자궁질량（0.15±0.05）g、자궁질량/체질량0.48±0.08]，차이균무통계학의의（균P＞0.05）；단여가수술조비교[자궁질량（0.45±0.08）g、자궁질량/체질량3.45±0.25]，차이균유통계학의의（균P＜0.01）。현미경하관찰대서자궁내막，거란소조자궁내막선체소이희소，사용불동제량GST적저、중급고제량조자궁내막잉여거란소조일양，분포불균균，선체내츤상피유립방혹편평세포조성，명현부우세포。결론불동제량GST균불회촉진거세대서자궁질량증가급자궁내막증생，재림상응용가능시안전적。
Objective To investigate the effect of different doses of genistein (GST)on uterus and endometrium in o-variectomized rats. Methods Forty female SD rats received ovariectomy and 10 rats received sham operation (normal control). The ovariectomized rats were further divided into 4 groups (10 in each):rats in low- dose, middle- dose and high- dose groups were fed with 25mg/kg·d, 50mg/kg·d and 100mg/kg·d GST, respectively; rats in overiectomized control group were fed with same volume of DMSO as those in normal control group. After 4 weeks, the animals were sacrificed, and uterus specimens were obtained. The ratio of weights of the uterine over body was calculated and histopathological examinations on the uterus were performed. Results After 4 weeks, body weights were increased in al groups, and there was no significant difference(P>0.05) among 5 groups. The weights of uterus in low, middle, high GST groups and overiectomized control group were 0.18±0.03g, 0.16±0.04g, 0.17±0.05g and 0.15±0.05g, respectively (P>0.05), and the uterine/body weight ratio was 0.64±0.05, 0.53± 0.08, 0.58±0.03 and 0.48±0.08, respectively (P>0.05). There were significant differences in uterus weight and uterus/body weight ratio between GST groups and normal control group (P<0.01). The histopathology showed that uterus from ovariec-tomized group and GST groups had smal er endometrial glands than those from normal rats, and there was uneven distribution of glands and the glands were lined by cuboidal or squamous epithelial cells. Conclusion Different doses of GST have no sig-nificant effect on uterus enlarging and endometrial hyperplasia in ovariectomized rats, suggesting that GST administration might be safe in clinical application.
