中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2014年
28期
11-13
,共3页
谢文剑%曹艺%柏玉冰%谢嘉驰%夏伯候%李亚梅%潘清平%林丽美
謝文劍%曹藝%柏玉冰%謝嘉馳%夏伯候%李亞梅%潘清平%林麗美
사문검%조예%백옥빙%사가치%하백후%리아매%반청평%림려미
夏枯草%熊果酸%超高效液相色谱法%含量测定
夏枯草%熊果痠%超高效液相色譜法%含量測定
하고초%웅과산%초고효액상색보법%함량측정
Prune lla v ulgaris L.%Ursolic acid%UPLC%Determination
目的：建立夏枯草药材中熊果酸含量的超高效液相色谱法(UPLC)测定方法。方法采用UPLC进行测定，色谱柱为ACQUITY UPLC BEH C18(2.1 mm×100 mm，1.7μm)，以甲醇-0.5%冰乙酸水为流动相进行等度洗脱，流速为0.3 ml/min，柱温30℃，检测波长210 nm。结果熊果酸在0.2461~0.4922 mg范围内呈良好的线性关系(r2=0.9999)，平均回收率为99.29%，R SD=1.74%，熊果酸在夏枯草中的含量为0.28%~0.53%。结论该方法快速、高效、重现性好，可以为夏枯草药材的质量控制提供更有效的手段。
目的：建立夏枯草藥材中熊果痠含量的超高效液相色譜法(UPLC)測定方法。方法採用UPLC進行測定，色譜柱為ACQUITY UPLC BEH C18(2.1 mm×100 mm，1.7μm)，以甲醇-0.5%冰乙痠水為流動相進行等度洗脫，流速為0.3 ml/min，柱溫30℃，檢測波長210 nm。結果熊果痠在0.2461~0.4922 mg範圍內呈良好的線性關繫(r2=0.9999)，平均迴收率為99.29%，R SD=1.74%，熊果痠在夏枯草中的含量為0.28%~0.53%。結論該方法快速、高效、重現性好，可以為夏枯草藥材的質量控製提供更有效的手段。
목적：건립하고초약재중웅과산함량적초고효액상색보법(UPLC)측정방법。방법채용UPLC진행측정，색보주위ACQUITY UPLC BEH C18(2.1 mm×100 mm，1.7μm)，이갑순-0.5%빙을산수위류동상진행등도세탈，류속위0.3 ml/min，주온30℃，검측파장210 nm。결과웅과산재0.2461~0.4922 mg범위내정량호적선성관계(r2=0.9999)，평균회수솔위99.29%，R SD=1.74%，웅과산재하고초중적함량위0.28%~0.53%。결론해방법쾌속、고효、중현성호，가이위하고초약재적질량공제제공경유효적수단。
Objective To establish an UPLC method for the determination of ursolic acid in Prunella vulgaris L.. Methods Ursolic acid was determined by UPLC.The assay was performed on a ACQUITY UPLC BEH C18 (2.1 mmí100 mm, 1.7 μm) column and eluted with a mobile phase consisted of methanol-0.5% acetic acid at a flow rate of 0.3 ml/min. The column temperature was 30℃.The detection wavelength was 210 nm. Results The standard curve was in good lin-earity over the range of 0.2461 to 0.4922 mg (r2=0.9999),the recovery of ursolic acid was 99.29% and RSD was 1.74%. The content of ursolic acid was in the range of 0.28%to 0.53%. Conclusion The method is simple,accurate and can be used to control the quality of ursolic acid in Prune lla v ulgaris L..
