江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2014年
9期
52-56
,共5页
生防放线菌MⅠ2%黄芩%防御酶
生防放線菌MⅠ2%黃芩%防禦酶
생방방선균MⅠ2%황금%방어매
Actinomycetes MⅠ2%Scutdlaria baicalensis%Defense enzyme
在高氏1号培养基中加入质量浓度为50μg/mL的化学抑制剂K2 Cr2 O7，用抑菌圈法从陕西省商州区土壤样品中筛选出8株放线菌，其中MⅠ2菌株对黄芩根腐病菌的抑制率最大（31．27％），可见MⅠ2菌株的生防效果最强。用放线菌MⅠ2发酵液诱导处理黄芩幼苗，测定在不同生长期黄芩叶片中防御酶系活性的变化。结果表明：MⅠ2菌株发酵液处理黄芩与CK相比，均能引起黄芩体内防御酶发生明显变化，过氧化氢酶（ CAT）的活性受到抑制，过氧化物酶（ POD）和苯丙氨酸解氨酶（ PAL）活性均有所提高。 CAT和POD活性以发酵液原液的作用效果最为显著，而PAL活性以发酵液稀释20倍的作用效果最为明显。放线菌MⅠ2菌株发酵液诱导黄芩使其防御酶系活性发生变化而提高植株的抗病性。
在高氏1號培養基中加入質量濃度為50μg/mL的化學抑製劑K2 Cr2 O7，用抑菌圈法從陝西省商州區土壤樣品中篩選齣8株放線菌，其中MⅠ2菌株對黃芩根腐病菌的抑製率最大（31．27％），可見MⅠ2菌株的生防效果最彊。用放線菌MⅠ2髮酵液誘導處理黃芩幼苗，測定在不同生長期黃芩葉片中防禦酶繫活性的變化。結果錶明：MⅠ2菌株髮酵液處理黃芩與CK相比，均能引起黃芩體內防禦酶髮生明顯變化，過氧化氫酶（ CAT）的活性受到抑製，過氧化物酶（ POD）和苯丙氨痠解氨酶（ PAL）活性均有所提高。 CAT和POD活性以髮酵液原液的作用效果最為顯著，而PAL活性以髮酵液稀釋20倍的作用效果最為明顯。放線菌MⅠ2菌株髮酵液誘導黃芩使其防禦酶繫活性髮生變化而提高植株的抗病性。
재고씨1호배양기중가입질량농도위50μg/mL적화학억제제K2 Cr2 O7，용억균권법종합서성상주구토양양품중사선출8주방선균，기중MⅠ2균주대황금근부병균적억제솔최대（31．27％），가견MⅠ2균주적생방효과최강。용방선균MⅠ2발효액유도처리황금유묘，측정재불동생장기황금협편중방어매계활성적변화。결과표명：MⅠ2균주발효액처리황금여CK상비，균능인기황금체내방어매발생명현변화，과양화경매（ CAT）적활성수도억제，과양화물매（ POD）화분병안산해안매（ PAL）활성균유소제고。 CAT화POD활성이발효액원액적작용효과최위현저，이PAL활성이발효액희석20배적작용효과최위명현。방선균MⅠ2균주발효액유도황금사기방어매계활성발생변화이제고식주적항병성。
In this study, 50μg/mL K2Cr2O7 was added into GA No.1 culture medium, and eight strains of actinomyces were selected out from the soil samples of Shangzhou district in Shaanxi province by using inhibition zone method .Among them , the strain MⅠ2 had the highest inhibition rate (31.27%) to the pathogen ( Fusarium sp.) of root rot in Scutdlaria baicalensis, showing the strongest biological control effect .The seedlings of Scutellaria baicalensis were induced and treated with actinomyces MⅠ2 fermenta-tion products , and the activity of defense enzyme system in the leaves of Scutellaria baicalensis at various growth stages was meas-ured.The results indicated that:in comparison with the control , the treatment with MⅠ2 fermentation products all could cause the obvious changes in the activity of defense enzymes in Scutellaria baicalensis, namely the activity of catalase (CAT) was inhibited, and that of both peroxidase ( POD) and phenylalanine ammonia -lyase ( PAL) was enhanced .The stoste of MⅠ2 fermentation liq-uid had the most significant promotive effect on the activity of CAT and POD , while the 20-fold dilution of MⅠ2 fermentation liq-uid had the most significant promotive effect on the activity of PAL .The fermentation products of actinomyces strain MⅠ2 increased the disease resistance of Scutdlaria baicalensis through inducing its defense enzyme activity to change .
