动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
9期
118-122,123
,共6页
南海辰%夏利宁%刘英玉%翟少华%底丽娜
南海辰%夏利寧%劉英玉%翟少華%底麗娜
남해신%하리저%류영옥%적소화%저려나
大肠埃希菌%抗菌药物%耐药%微量肉汤法
大腸埃希菌%抗菌藥物%耐藥%微量肉湯法
대장애희균%항균약물%내약%미량육탕법
Escherichia coli%antimicrobial%drug resistance%broth method
为了解新疆塔城某规模化养殖场分离的大肠埃希菌对临床常用抗菌药物的耐药情况,从该规模化养殖场中采集的水样、饲料样、牛粪样及羊粪样中分离大肠埃希菌。采用微量肉汤法检测其对抗菌药物的耐药情况。结果表明,采集牛源饮用水样35份,分离率100.0%(35/35),分离的大肠埃希菌仅对阿莫西林/克拉维酸(31.4%)和氨苄西林(20.0%)2种抗菌药物耐药;牛源饲料样15份,分离率86.7%(13/15),分离的大肠埃希菌对氨苄西林(30.8%)、阿莫西林/克拉维酸(23.1%)、安普霉素(15.4%)、诺氟沙星(7.7%)、恩诺沙星(7.7%)和庆大霉素(7.7%)6种抗菌药物耐药;牛粪样20份,分离率100.0%(20/20),分离的大肠埃希菌对氨苄西林(60.0%)、阿莫西林/克拉维酸(50.0%)、恩诺沙星(40.0%)、庆大霉素(40.0%)、头孢噻呋(35.0%)、阿米卡星(25.0%)、诺氟沙星(10.0%)和环丙沙星(10.0%)8种抗菌药物耐药;羊粪样55份,分离率100.0%(55/55),分离的大肠埃希菌对阿莫西林/克拉维酸(25.5%)、氨苄西林(12.7%)、庆大霉素(5.5%)、头孢噻呋(3.6%)、诺氟沙星(1.8%)、恩诺沙星(1.8%)和阿米卡星(1.8%)7种抗菌药物耐药。新疆塔城牛源大肠埃希菌对常用抗菌药物多药耐药情况较严重,临床用药需谨慎,且可能存在粪源菌污染水源和饲料的风险。
為瞭解新疆塔城某規模化養殖場分離的大腸埃希菌對臨床常用抗菌藥物的耐藥情況,從該規模化養殖場中採集的水樣、飼料樣、牛糞樣及羊糞樣中分離大腸埃希菌。採用微量肉湯法檢測其對抗菌藥物的耐藥情況。結果錶明,採集牛源飲用水樣35份,分離率100.0%(35/35),分離的大腸埃希菌僅對阿莫西林/剋拉維痠(31.4%)和氨芐西林(20.0%)2種抗菌藥物耐藥;牛源飼料樣15份,分離率86.7%(13/15),分離的大腸埃希菌對氨芐西林(30.8%)、阿莫西林/剋拉維痠(23.1%)、安普黴素(15.4%)、諾氟沙星(7.7%)、恩諾沙星(7.7%)和慶大黴素(7.7%)6種抗菌藥物耐藥;牛糞樣20份,分離率100.0%(20/20),分離的大腸埃希菌對氨芐西林(60.0%)、阿莫西林/剋拉維痠(50.0%)、恩諾沙星(40.0%)、慶大黴素(40.0%)、頭孢噻呋(35.0%)、阿米卡星(25.0%)、諾氟沙星(10.0%)和環丙沙星(10.0%)8種抗菌藥物耐藥;羊糞樣55份,分離率100.0%(55/55),分離的大腸埃希菌對阿莫西林/剋拉維痠(25.5%)、氨芐西林(12.7%)、慶大黴素(5.5%)、頭孢噻呋(3.6%)、諾氟沙星(1.8%)、恩諾沙星(1.8%)和阿米卡星(1.8%)7種抗菌藥物耐藥。新疆塔城牛源大腸埃希菌對常用抗菌藥物多藥耐藥情況較嚴重,臨床用藥需謹慎,且可能存在糞源菌汙染水源和飼料的風險。
위료해신강탑성모규모화양식장분리적대장애희균대림상상용항균약물적내약정황,종해규모화양식장중채집적수양、사료양、우분양급양분양중분리대장애희균。채용미량육탕법검측기대항균약물적내약정황。결과표명,채집우원음용수양35빈,분리솔100.0%(35/35),분리적대장애희균부대아막서림/극랍유산(31.4%)화안변서림(20.0%)2충항균약물내약;우원사료양15빈,분리솔86.7%(13/15),분리적대장애희균대안변서림(30.8%)、아막서림/극랍유산(23.1%)、안보매소(15.4%)、낙불사성(7.7%)、은낙사성(7.7%)화경대매소(7.7%)6충항균약물내약;우분양20빈,분리솔100.0%(20/20),분리적대장애희균대안변서림(60.0%)、아막서림/극랍유산(50.0%)、은낙사성(40.0%)、경대매소(40.0%)、두포새부(35.0%)、아미잡성(25.0%)、낙불사성(10.0%)화배병사성(10.0%)8충항균약물내약;양분양55빈,분리솔100.0%(55/55),분리적대장애희균대아막서림/극랍유산(25.5%)、안변서림(12.7%)、경대매소(5.5%)、두포새부(3.6%)、낙불사성(1.8%)、은낙사성(1.8%)화아미잡성(1.8%)7충항균약물내약。신강탑성우원대장애희균대상용항균약물다약내약정황교엄중,림상용약수근신,차가능존재분원균오염수원화사료적풍험。
In order to investigate commonly used antimicrobial drug resistance to Escherichia coli isolates from a farm in Tacheng,Xinj iang,the minimal inhibitory concentrations (MIC)of the antimicrobial drugs to these isolates from drinking water,feed,bovine feces and ovine feces were determined by the broth mi-cro-dilution method.The results showed that:3 5 E.coli isolates were confirmed from 3 5 drinking water samples (100.0%),the resistance rates to amoxicillin/clavulanic acid and ampicillin were 31.4% and 20%,respectively.13 E.coli isolates were confirmed from 15 feed samples (86.7%),the resistance rates to ampicillin, amoxicillin/clavulanic acid, apramycin, norfloxacin, enrofloxacin and gentamicin were 30.8%,23.1%,15.4%,7.7%,7.7% and 7.7%,respectively.20 E.coli isolates were confirmed from 20 bovine fecals samples(100.0%),the resistance rates to ampicillin,amoxicillin/clavulanic acid,enro-floxacin,gentamicin,ceftiofur,amikacin,norfloxacin and ciprofloxacin were 60.0%,50.0%,40.0%, 40.0%,35.0%,25.0%,10.0% and 10.0%,respectively.55 E.coli isolates were confirmed from 55 o-vine fecals samples(100.0%),the resistance rates to amoxicillin/clavulanic acid,ampicillin,gentamicin, ceftiofur,norfloxacin,enrofloxacin and amikacin were 25.5%,12.7%,5.5%,3.6%,1.8%,1.8% and 1.8%,respectively.The multi-drug resistance of E.coli is serious in Tacheng Xinjiang.The clinical medi-cation needs to be careful,and there may be a risk of fecal bacterial pollution to water and feed.