CAJ | 학술논문

牛呼吸道合胞体病毒是引起牛呼吸道疾病的主要病原之一。进行牛呼吸道合胞体病诊断时，首先通过临床症状观察以及病理剖检变化进行初诊，然后再进行实验室诊断。其实验室检测主要依赖于病原学诊断和血清学诊断，病原学诊断方法主要包括细胞分离培养鉴定、聚合酶链反应。血清学方法包括中和试验、免疫荧光试验、酶联免疫吸附试验等。近年来聚合酶链反应！酶联免疫吸附试验等方法得到快速发展，凭借其高效、快速、灵敏性高的特点成为牛呼吸道合胞体病毒检测的常用方法。牛呼吸道合胞体病在全球范围内流行，对各国养牛业造成极大危害。论文综述了牛呼吸道合胞体病毒检测方法的研究进展，为牛呼吸道合胞体病的诊断和预防提供参考。
우호흡도합포체병독시인기우호흡도질병적주요병원지일。진행우호흡도합포체병진단시，수선통과림상증상관찰이급병리부검변화진행초진，연후재진행실험실진단。기실험실검측주요의뢰우병원학진단화혈청학진단，병원학진단방법주요포괄세포분리배양감정、취합매련반응。혈청학방법포괄중화시험、면역형광시험、매련면역흡부시험등。근년래취합매련반응！매련면역흡부시험등방법득도쾌속발전，빙차기고효、쾌속、령민성고적특점성위우호흡도합포체병독검측적상용방법。우호흡도합포체병재전구범위내류행，대각국양우업조성겁대위해。논문종술료우호흡도합포체병독검측방법적연구진전，위우호흡도합포체병적진단화예방제공삼고。
Bovine respiratory syncytial virus is recognized as one of the crucial causes of bovine respiratory disease,which has a marked impact on the cattle industry and the dairy industry.Bovine respiratory syncy-tial virus is preliminarily diagnosed based on the clinical symptoms and pathological anatomy changes,and then through the laboratory tests.The laboratory tests of bovine respiratory syncytial virus mainly rely on etiology diagnosis and serological diagnosis.The methods for etiology diagnosis consists of cell-culture iso-lation techniques,polymerase chain reaction.And the serological methods consists of neutralization tests, immunofluorescence method,enzyme linked immunosorbent assay.For the past few years,the experimen-tal methods,such as polymerase chain reaction and enzyme-linked immunosorbent assay were developed rapidly,and became the main methods for the diagnosis of bovine respiratory syncytial virus due to their high efficiency,rapidness and high sensitivity.The bovine respiratory syncytial disease has spread world-wide and impacted production and animal welfare in the cattle industry.The article summarized the re-search progress on the laboratory test methods of bovine respiratory syncytial virus.The overview of thesis will provide some references for the diagnosis and prevention of the bovine respiratory syncytial disease.