CAJ | 학술논문

采用细胞化学和酶联免疫吸附试验（ELISA）探讨维生素 E 对炔雌醚致小鼠生殖毒性的保护作用。将15只8周龄雄性昆明小鼠随机分为3组，适应饲养1周后分别灌胃橄榄油、橄榄油＋炔雌醚、橄榄油＋炔雌醚＋维生素 E，每天1次，连续3周。处理结束后，睾丸、附睾、精囊腺及前列腺称重；取睾丸，测定抗氧化能力；取附睾，检测精子品质。结果显示，1 mg/kg 炔雌醚组显著降低生殖器官的重量，抑制器官发育；降低抗氧化酶活性、升高活性氧含量，破坏抗氧化能力；造成精子品质下降。100 mg/kg维生素 E 组显著升高生殖器官重量、抗氧化酶活性及精子品质。推测增强抗氧化酶活性是维生素 E 对生殖功能保护作用的途径之一，其深层机制有待进一步研究。
채용세포화학화매련면역흡부시험（ELISA）탐토유생소 E 대결자미치소서생식독성적보호작용。장15지8주령웅성곤명소서수궤분위3조，괄응사양1주후분별관위감람유、감람유＋결자미、감람유＋결자미＋유생소 E，매천1차，련속3주。처리결속후，고환、부고、정낭선급전렬선칭중；취고환，측정항양화능력；취부고，검측정자품질。결과현시，1 mg/kg 결자미조현저강저생식기관적중량，억제기관발육；강저항양화매활성、승고활성양함량，파배항양화능력；조성정자품질하강。100 mg/kg유생소 E 조현저승고생식기관중량、항양화매활성급정자품질。추측증강항양화매활성시유생소 E 대생식공능보호작용적도경지일，기심층궤제유대진일보연구。
This study aimed to investigate the protective effect of vitamin E on quinestrol induced reproduc-tive toxicity in mice by using the cell chemistry and enzyme linked immunosorbent assay (ELISA)method. 15 8-week old male Kunming mice were randomly divided into 3 groups and adaptively feed for 1 week,and then treated by intragastric administration of 50 μL olive oil containing 0.1 mg/kg quinestrol,1 mg/kg quinestrol+100 mg/kg vitamin E body weight respectively,1 times a day,for 2 consecutive weeks.At the end of treatment,testes,epididymes and gonads were separated and weighed;testes were treated for an-tioxidant activity assay;epididymes were treated for sperm quality assay.The results showed that 1 mg/kg quinestrol significantly reduced reproductive organ weights,inhibited organ development;reduced the ac-tivity of antioxidant enzymes,increased content of active oxygen damage;declined the sperm quality.100 mg/kg vitamin E significantly increased the weight of reproductive organs,antioxidase activity and sperm quality.The results indicated that increasing antioxidase activity is one of the ways of the protective effects of vitamin E on reproductive function,and the underlying mechanism remains to be further studied.