CAJ | 학술논문

对分离的血红蛋白抗菌肽进行生物活性鉴定。通过微量稀释法测定血红蛋白抗菌肽对大肠埃希菌、金黄色葡萄球菌、白色念珠菌的最小抑菌浓度（MIC）；以血红蛋白释放量测定其溶血性；琼脂平皿试验测定其热稳定性；并进一步在扫描电镜（SEM）下观察血红蛋白抗菌肽对测试菌株形态结构的影响。结果表明，血红蛋白抗菌肽对大肠埃希菌、金黄色葡萄球菌和白色念珠菌的 MIC 分别为0．5、1．0、0．25 mg/mL；在2．0 mg/mL 的浓度下，可造成6．7％的红细胞发生溶血；经过高温处理，仍表现出较好的热稳定性；SEM 观察到血红蛋白抗菌肽作用的细菌形态发生严重的皱缩、变形并大量的聚集，细胞膜裂解、破碎。说明血红蛋白抗菌肽具有良好的抗菌活性且溶血性较低，具有良好的生物安全性。
대분리적혈홍단백항균태진행생물활성감정。통과미량희석법측정혈홍단백항균태대대장애희균、금황색포도구균、백색념주균적최소억균농도（MIC）；이혈홍단백석방량측정기용혈성；경지평명시험측정기열은정성；병진일보재소묘전경（SEM）하관찰혈홍단백항균태대측시균주형태결구적영향。결과표명，혈홍단백항균태대대장애희균、금황색포도구균화백색념주균적 MIC 분별위0．5、1．0、0．25 mg/mL；재2．0 mg/mL 적농도하，가조성6．7％적홍세포발생용혈；경과고온처리，잉표현출교호적열은정성；SEM 관찰도혈홍단백항균태작용적세균형태발생엄중적추축、변형병대량적취집，세포막렬해、파쇄。설명혈홍단백항균태구유량호적항균활성차용혈성교저，구유량호적생물안전성。
To analyze the bioactivity of novel hemoglobin antimicrobial peptide,the minimal inhibitory con-centration of hemoglobin antimicrobial peptide to Escherichia coli ,Staphylococcus aureus,Monilia albican was tested by broth microdilution method.The hemolysis was tested by measuring the release of hemoglo-bin.Thermal stability of hemoglobin antimicrobial peptide was determined by agar gel diffusion method. And the morphology of bacteria tesed were observed by scanning electron microscope.The results showed that the MIC to Escherichia coli ,Staphylococcus aureus ,Monilia albican were 0.5,1.0,0.25 mg/mL. It caused 6.7% rupture of erythrocyte at the concentration of 2.0 mg/mL.After heated,it kept stability partly.The shrinking,distorting and gathering of microorganism were observed.It indicated that hemo-globin antimicrobial peptide had a good bio-safety in the development and application,which had a signifi-cant antibacterial activity and low hemolysis.