动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
9期
46-51
,共6页
张永红%官佳懿%崔德凤%谷崇高%徐双%李洋%沈红
張永紅%官佳懿%崔德鳳%穀崇高%徐雙%李洋%瀋紅
장영홍%관가의%최덕봉%곡숭고%서쌍%리양%침홍
绿原酸%巨噬细胞%增殖%分泌%吞噬%小鼠
綠原痠%巨噬細胞%增殖%分泌%吞噬%小鼠
록원산%거서세포%증식%분비%탄서%소서
chlorogenic acid%macrophage%proliferation%secretion%phagoeytosis%mouse
为观察绿原酸（CHA）对小鼠不同组织巨噬细胞功能的影响，采用无菌操作制备小鼠腹腔、骨髓和脾脏巨噬细胞，体外培养在含有脂多糖（LPS）和绿原酸的 RPMI1640培养液中，用 MTT 法检测细胞增殖，ELISA 和 Griess 法分别分析细胞分泌 TNF-α和 NO 的水平，瑞氏-吉姆萨染色法测定巨噬细胞吞噬能力。结果与对照组和 LPS 组相比，腹腔、脾脏和骨髓3种不同组织巨噬细胞 CHA 组和 LPS 联合 CHA 组 A值均有所降低，即3种巨噬细胞增殖转化率均有所下降，3种不同组织巨噬细胞 CHA 组和 CHA＋LPS 组NO 分泌量明显增加，且具有剂量依赖性。较之 LPS 组，不同组织巨噬细胞 CHA 组 TNF-α分泌量降低，而脾脏和骨髓巨噬细胞 LPS 联合 CHA 组分泌 TNF-α量显著提高，腹腔巨噬细胞组不显著。CHA 显著提高LPS 刺激的腹腔巨噬细胞吞噬能力。此外，CHA 可显著提高环磷酰胺处理小鼠脾脏和骨髓巨噬细胞存活率，但使脾脏和腹腔巨噬细胞分泌 NO 量显著减少。结果提示绿原酸对小鼠不同组织巨噬细胞增殖、TNF-α和 NO 分泌以及吞噬能力均有不同程度影响。
為觀察綠原痠（CHA）對小鼠不同組織巨噬細胞功能的影響，採用無菌操作製備小鼠腹腔、骨髓和脾髒巨噬細胞，體外培養在含有脂多糖（LPS）和綠原痠的 RPMI1640培養液中，用 MTT 法檢測細胞增殖，ELISA 和 Griess 法分彆分析細胞分泌 TNF-α和 NO 的水平，瑞氏-吉姆薩染色法測定巨噬細胞吞噬能力。結果與對照組和 LPS 組相比，腹腔、脾髒和骨髓3種不同組織巨噬細胞 CHA 組和 LPS 聯閤 CHA 組 A值均有所降低，即3種巨噬細胞增殖轉化率均有所下降，3種不同組織巨噬細胞 CHA 組和 CHA＋LPS 組NO 分泌量明顯增加，且具有劑量依賴性。較之 LPS 組，不同組織巨噬細胞 CHA 組 TNF-α分泌量降低，而脾髒和骨髓巨噬細胞 LPS 聯閤 CHA 組分泌 TNF-α量顯著提高，腹腔巨噬細胞組不顯著。CHA 顯著提高LPS 刺激的腹腔巨噬細胞吞噬能力。此外，CHA 可顯著提高環燐酰胺處理小鼠脾髒和骨髓巨噬細胞存活率，但使脾髒和腹腔巨噬細胞分泌 NO 量顯著減少。結果提示綠原痠對小鼠不同組織巨噬細胞增殖、TNF-α和 NO 分泌以及吞噬能力均有不同程度影響。
위관찰록원산（CHA）대소서불동조직거서세포공능적영향，채용무균조작제비소서복강、골수화비장거서세포，체외배양재함유지다당（LPS）화록원산적 RPMI1640배양액중，용 MTT 법검측세포증식，ELISA 화 Griess 법분별분석세포분비 TNF-α화 NO 적수평，서씨-길모살염색법측정거서세포탄서능력。결과여대조조화 LPS 조상비，복강、비장화골수3충불동조직거서세포 CHA 조화 LPS 연합 CHA 조 A치균유소강저，즉3충거서세포증식전화솔균유소하강，3충불동조직거서세포 CHA 조화 CHA＋LPS 조NO 분비량명현증가，차구유제량의뢰성。교지 LPS 조，불동조직거서세포 CHA 조 TNF-α분비량강저，이비장화골수거서세포 LPS 연합 CHA 조분비 TNF-α량현저제고，복강거서세포조불현저。CHA 현저제고LPS 자격적복강거서세포탄서능력。차외，CHA 가현저제고배린선알처리소서비장화골수거서세포존활솔，단사비장화복강거서세포분비 NO 량현저감소。결과제시록원산대소서불동조직거서세포증식、TNF-α화 NO 분비이급탄서능력균유불동정도영향。
In order to investigate effects of chlorogenic acid (CHA)on mice macrophages of different tis-sues,mouse peritoneal,bone marrow and spleen macrophages by aseptic preparation were cultured in vitro and stimulated with lipopolysaccharide (LPS)in RPMI1640 medium containing CHA.MTT assay,ELISA and Griess methods were used to analyze cell proliferation,cell secretion levels of TNF-α and NO,and phagocytic activity of macrophages was detected by Wright-Giemsa staining.The results showed that there were significant decreases in A values and NO secretion levels of peritoneal,spleen and bone marrow mac-rophages in CHA groups and CHA+LPS groups compared with CHA groups and CHA+LPS groups,and there was a dose-dependent increase.Compared with LPS groups,the levels of TNF-αsecreted from mac-rophages of different tissues showed a decrease.There was a significant increase in TNF-α secretion of spleen and bone marrow macrophages in LPS+CHA groups,and not significant in that in peritoneal mac-rophages.CHA significantly enhanced phagocytic capacity of LPS-stimulated peritoneal macrophages.In addition,CHA significantly improved the survival of spleen and bone marrow macrophages from mouse treated with cyclophosphamide (CY),and there was a significant reduction in the amount of NO secreted from spleen and peritoneal macrophages.The results indicated that there were some degree effects of CHA on different tissue macrophage proliferation,TNF-α and NO secretion,and phagocytic activity of the mouse.
