CAJ | 학술논문

采用高效液相色谱串联质谱法测定鲫鱼血浆中喹乙醇的浓度。选用健康鲫鱼，随机分成2组，每组20尾，以30 mg/kg体重的剂量经口灌服给药，血浆用30 g/L的三氯乙酸沉淀蛋白后测定其浓度，统计软件3P97处理血药浓度-时间数据。鲫鱼经口灌服喹乙醇后的数据符合一级吸收一室模型，主要药动学参数：T1/2kα为1．09 h±0．14 h；T1/2Ke为5．97 h±1．06 h；Tmax为3．25 h±0．41 h；Cmax为25．73 mg/L±2．53 mg/L；AUC为325．35 mg/（L·h）-1±69．01。鲫鱼口服喹乙醇吸收迅速完全，消除较慢。
채용고효액상색보천련질보법측정즉어혈장중규을순적농도。선용건강즉어，수궤분성2조，매조20미，이30 mg/kg체중적제량경구관복급약，혈장용30 g/L적삼록을산침정단백후측정기농도，통계연건3P97처리혈약농도-시간수거。즉어경구관복규을순후적수거부합일급흡수일실모형，주요약동학삼수：T1/2kα위1．09 h±0．14 h；T1/2Ke위5．97 h±1．06 h；Tmax위3．25 h±0．41 h；Cmax위25．73 mg/L±2．53 mg/L；AUC위325．35 mg/（L·h）-1±69．01。즉어구복규을순흡수신속완전，소제교만。
The concentration of olaquindox in the plasma of Carassius auratus was determined by high per-formance liquid chromatography tandem mass spectrometry (LC-MS/MS).Healthy Carassius auratus, were randomly divided into 2 groups,20 tail each group of ,30 mg per kg body weight oral dose of olaquin-dox was used,the blood samples were collected.The plasma was treated with 30 mL/L trichloroacetic acid concentrations for precipitation and detection of protein,statistical software 3P97 was used for processing plasma concentration-time data.It's best to fit the olaquindox concentration-time data to one-compartment open model with first order absorption after single oral dosing.The pharmacokinetic parameters of ola-quindox in Carassiusauratus are as follows :the elimination half-life T1/2Ke is 5.97 h±1.06 h ,the distri-bution half-life T1/2ka is 1.09 h±0.14 h ,the peak time Tmax is 3.25 h±0.41 h ,the peak concentration CTmax is 25.73 mg/L±2.53 mg/L,and the area under the curve (AUC)is 325.35 mg/(L·h)-1 ±69.01 mg/(L·h)-1 .The results showed that olaquindox was rapidly and completely absorbed and slowly elimi-nated after oral administration to Carassius auratus.