CAJ | 학술논문

目的：探讨P16与Ki-67在恶性黑色素瘤和皮内痣中的表达及鉴别诊断意义。方法选取该院2005年5月至2012年8月病理诊断为恶性黑色素瘤的患者31例，所有患者由2位主任医师再次复查确诊。同时选取30例皮内痣患者作为对照。应用免疫组织化学法检测恶性黑色素瘤及皮内痣组织中P16与Ki-67蛋白表达情况，并分析其与临床病理参数的关系。结果在恶性黑色素瘤组织中P16与Ki-67蛋白阳性表达率与皮内痣组织比较，差异有统计学意义（P<0.05）；P16蛋白的阳性表达与浸润深度及淋巴结转移有关（P<0.05），与其他病理参数无关（P＞0.05）；Ki-67蛋白的阳性表达与临床病理参数均无关（P＞0.05）。P16的表达与Ki-67的表达呈负相关（r=-0.453，P<0.05）。结论联合检测P16与Ki-67对恶性黑色素瘤和皮内痣的鉴别诊断具有重要的意义。
목적：탐토P16여Ki-67재악성흑색소류화피내지중적표체급감별진단의의。방법선취해원2005년5월지2012년8월병리진단위악성흑색소류적환자31례，소유환자유2위주임의사재차복사학진。동시선취30례피내지환자작위대조。응용면역조직화학법검측악성흑색소류급피내지조직중P16여Ki-67단백표체정황，병분석기여림상병리삼수적관계。결과재악성흑색소류조직중P16여Ki-67단백양성표체솔여피내지조직비교，차이유통계학의의（P<0.05）；P16단백적양성표체여침윤심도급림파결전이유관（P<0.05），여기타병리삼수무관（P＞0.05）；Ki-67단백적양성표체여림상병리삼수균무관（P＞0.05）。P16적표체여Ki-67적표체정부상관（r=-0.453，P<0.05）。결론연합검측P16여Ki-67대악성흑색소류화피내지적감별진단구유중요적의의。
Objective To explore the expression and diagnostic value of P16 and Ki-67 in malignant melanoma and in-tradermal nevus. Methods Thirty-one patients,who were diagnosed with malignant melanoma in the hospital from May 2005 to August 2012, were selected and reexamined by two chief physicians. Meanwhile other 30 patients with intradermal nevus were selected as control group. Immunohistochemical method was used to detect the protein expression of P16 and Ki-67 in malignant melanoma and intradermal nerus ,and analyze the relationship with clnicopathological parameters. Results There were statisti-cally significant differences in positive rate of P16 protein and Ki-67 protein between malignant melanoma tissue and intradermal nerus tissue(P<0.05). The expression of P16 protein was correlated with depth of invasion and lymphatic metastasis (P<0.05), but there was no correlation between expression of P16 protein and other pathological parameters (P>0.05). There was no correlation between expression of Ki-67 protein and other clinicopathological parameters (P>0.05). There was a negative correlation between P16 expression and Ki-67 expression(r=-0.453,P<0.05). Conclusion Combined detection of P16 and Ki-67 has vital signifi-cance for the differential diagnosis of malignant melanoma and benign nevus melanin.