中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
17期
3133-3136
,共4页
脊髓损伤%细胞凋亡%leydig细胞%PARP%Fas
脊髓損傷%細胞凋亡%leydig細胞%PARP%Fas
척수손상%세포조망%leydig세포%PARP%Fas
Spinal cord injuries%Apoptosis%Leydig cell%PARP%Fas
目的：研究切除精索神经对Leydig细胞凋亡和PARP、Fas基因表达的影响。方法成年健康SD大鼠20只，随机分为假手术组和脊髓损伤组，手术后14 d以Percoll连续密度梯度法提取Leydig细胞后采用流式细胞仪（FACS）定量检测Leydig细胞凋亡情况，以Western blot方法检测Leydig细胞中cleaved-PARP、Fas蛋白的表达。结果脊髓损伤组凋亡Leydig细胞比例（32.15%）显著高于假手术组（12.78%）（P＜0.05），其Fas蛋白及Cleaved-PARP蛋白的表达水平均显著升高。结论脊髓损伤诱发Leydig细胞凋亡，PARP、Fas基因参与对该过程的调控。
目的：研究切除精索神經對Leydig細胞凋亡和PARP、Fas基因錶達的影響。方法成年健康SD大鼠20隻，隨機分為假手術組和脊髓損傷組，手術後14 d以Percoll連續密度梯度法提取Leydig細胞後採用流式細胞儀（FACS）定量檢測Leydig細胞凋亡情況，以Western blot方法檢測Leydig細胞中cleaved-PARP、Fas蛋白的錶達。結果脊髓損傷組凋亡Leydig細胞比例（32.15%）顯著高于假手術組（12.78%）（P＜0.05），其Fas蛋白及Cleaved-PARP蛋白的錶達水平均顯著升高。結論脊髓損傷誘髮Leydig細胞凋亡，PARP、Fas基因參與對該過程的調控。
목적：연구절제정색신경대Leydig세포조망화PARP、Fas기인표체적영향。방법성년건강SD대서20지，수궤분위가수술조화척수손상조，수술후14 d이Percoll련속밀도제도법제취Leydig세포후채용류식세포의（FACS）정량검측Leydig세포조망정황，이Western blot방법검측Leydig세포중cleaved-PARP、Fas단백적표체。결과척수손상조조망Leydig세포비례（32.15%）현저고우가수술조（12.78%）（P＜0.05），기Fas단백급Cleaved-PARP단백적표체수평균현저승고。결론척수손상유발Leydig세포조망，PARP、Fas기인삼여대해과정적조공。
Objective To explore influence of spinal cord injury on leydig cell apoptosis and expression of PARP and Fas gene.Methods20 adult healthy SD rats were randomly divided into sham-operation group and spinal cord injury (SCI) group. The animals were killed on 14 days after operation. Fluorescence activated cell sorter (FACS) was used to quantitatively assess apoptosis of Leydig cells. Western-blot was applied to detect cleaved-PARP and Fas proteins.Results On day 14 after operation, the incidence of leydig cell apoptosis in SCI group was 32.15%, significantly higher than that of sham-operation group (12.78%). Cleaved-PARP and Fas proteins were both found dramatically higher in SCI group than sham-operation group.Conclusion Spinal cord injury triggers apoptosis of Leydig cells, and PARP and Fas gene are involved in regulation of this process.
