CAJ | 학술논문

目的：分析比较酶联免疫法（ELISA）和间接血凝法（IHA）在本实验室检测实验用家兔弓形虫特异性抗体的可行性。方法应用ELISA和IHA两种方法平行检测实验感染弓形虫的12只家兔阳性血清和未感染过弓形虫的30只正常家兔阴性血清中的弓形虫特异性抗体。比较两种方法敏感性、特异性、检测效率及Youden指数并运用kappa值进行一致性的评价。结果 IHA法的敏感性41.67%，特异性93.33%，ELISA法的敏感性100%，特异性100%。ELISA方法的敏感性、特异性、检测效率及Youden指数都在IHA方法之上。两种方法的总符合率是78.57%。kappa值=0.3998。结论 ELISA法与IHA法在本次平行检测中的一致性较差。ELISA方法敏感性、特异性明显优于IHA方法，更适用于实验动物家兔的弓形虫特异性抗体的检测。
목적：분석비교매련면역법（ELISA）화간접혈응법（IHA）재본실험실검측실험용가토궁형충특이성항체적가행성。방법응용ELISA화IHA량충방법평행검측실험감염궁형충적12지가토양성혈청화미감염과궁형충적30지정상가토음성혈청중적궁형충특이성항체。비교량충방법민감성、특이성、검측효솔급Youden지수병운용kappa치진행일치성적평개。결과 IHA법적민감성41.67%，특이성93.33%，ELISA법적민감성100%，특이성100%。ELISA방법적민감성、특이성、검측효솔급Youden지수도재IHA방법지상。량충방법적총부합솔시78.57%。kappa치=0.3998。결론 ELISA법여IHA법재본차평행검측중적일치성교차。ELISA방법민감성、특이성명현우우IHA방법，경괄용우실험동물가토적궁형충특이성항체적검측。
ObjectiveTo analyze and compare the feasibility of using ELISA and IHA in our laboratory for testing specific TOX-Ab in laboratory rabbit.Methods Used ELISA and IHA methods for a parallelized testing of the specific TOX-Ab contained in both the positive serum of 12 rabbits infected with toxoplasma gondii and negative serum of 30 uninfected rabbits, thus to compare the sensibility, specificity, testing efficiency and Youden index between the two methods before applying Kappa values for a consistency evaluation.Results The sensibility and specificity of IHA was 41.67% and 93.33% respectively, while that for ELISA are both 100%. The sensibility, specificity, testing efficiency and Youden index for ELISA are higher than that of IHA. The total consistency rate for such two methods was 78.57% with Kappa value equals to 0.3998.Conclusion The consistency between the use of ELISA and IHA for this parallelized testing is relatively poor, and ELISA is notably better than IHA in terms of sensitivity and specificity, therefore it is more suitable to be applied in the testing of the specific TOX-Ab in laboratory animal-rabbit.