CAJ | 학술논문

本试验采集10份代表性菜籽粕样品，选用28～35日龄科宝肉仔鸡，采用全收粪法、指示剂法、回肠食糜法3种试验方法，结合套算法测定菜籽粕的表观代谢能（AME）、氮校正表观代谢能（AMEn）和回肠表观消化能（IDE），并评估有效能值的变异。从上述结果中选取有效能值高、中、低的3个菜籽粕样品，进一步评估添加复合酶制剂对其有效能值的影响。结果表明，10个不同来源菜籽粕间AME和AMEn存在较大变异，变异范围分别为6.73～10.01 MJ/kg和6.62～9.81 MJ/kg。指示剂法和回肠食糜法测得的菜籽粕有效能值相当，均显著低于全收粪法测定值（P＜0.05）。三种测定方法测得的有效能值变异系数为10.04%～10.93%。与对照组相比，添加两种复合酶制剂（A和B）均可提高菜籽粕的AME、AMEn和IDE，但差异均不显著（P＞0.05）。酶制剂B对菜籽粕有效能值的提高幅度略高于酶制剂A，且品质较差的菜籽粕添加酶制剂的效果优于品质好的。
본시험채집10빈대표성채자박양품，선용28～35일령과보육자계，채용전수분법、지시제법、회장식미법3충시험방법，결합투산법측정채자박적표관대사능（AME）、담교정표관대사능（AMEn）화회장표관소화능（IDE），병평고유효능치적변이。종상술결과중선취유효능치고、중、저적3개채자박양품，진일보평고첨가복합매제제대기유효능치적영향。결과표명，10개불동래원채자박간AME화AMEn존재교대변이，변이범위분별위6.73～10.01 MJ/kg화6.62～9.81 MJ/kg。지시제법화회장식미법측득적채자박유효능치상당，균현저저우전수분법측정치（P＜0.05）。삼충측정방법측득적유효능치변이계수위10.04%～10.93%。여대조조상비，첨가량충복합매제제（A화B）균가제고채자박적AME、AMEn화IDE，단차이균불현저（P＞0.05）。매제제B대채자박유효능치적제고폭도략고우매제제A，차품질교차적채자박첨가매제제적효과우우품질호적。
Ten representative rapeseed meal (RSM)samples were collected in this trial,and three kinds of biological methods including total feces collection,indicator and ileal digesta were used,to analyze apparent metabolizable energy (AME),nitrogen corrected apparent metabolizable energy (AMEn)and apparent ileal digestible energy (IDE)by Cobb broilers at 28 ~ 35 day old,and also to assess the variability of available energy.Based on the above results,three RSM samples labeled high,medium and low at available energy were selected to estimate the effect of enzyme preparation sup-plementation on available energy of RSM.The results showed that huge variation at AME and AMEn of ten RSM were found,and the value of them was 6.73 ~ 10.01 MJ/kg and 6.62 ~ 9.81 MJ/kg,respectively.The results measured by total feces collection method were significantly lower than that of values obtained by indicator method and ileal digesta (P <0.05).Three measurement methods could be effectively used in determination of available energy of RSM,and the coeffi-cient of variation was 10.04% ~10.93%.Compared with control group,AME,AMEn and IDE were improved by both com-plex enzyme A and B supplementation (P > 0.05),and the enzyme B was better than enzyme A,and the RSM with poor quality would be effectively improved by enzyme supplementation.