国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
17期
2354-2355,2358
,共3页
王冰%李迎慧%扈庆华%林一曼%石晓路%邱亚群%贺连华%陈妙玲%吴平芳%许舒乐
王冰%李迎慧%扈慶華%林一曼%石曉路%邱亞群%賀連華%陳妙玲%吳平芳%許舒樂
왕빙%리영혜%호경화%림일만%석효로%구아군%하련화%진묘령%오평방%허서악
金黄色葡萄球菌%熔解曲线%分子分型
金黃色葡萄毬菌%鎔解麯線%分子分型
금황색포도구균%용해곡선%분자분형
Staphylococcus aureus%resolution melting%molecular typing
目的：建立金黄色葡萄球菌熔解曲线分子分型体系，应用于金黄色葡萄球菌食物中毒快速溯源。方法根据文献合成金黄色葡萄球菌 VNTR 位点的相应引物，用于检测已知 PFGE 型别的金黄色葡萄球菌，从中筛选出4个分辨力较高的位点。在此基础上建立熔解曲线分子分型体系，检测59株分离自食物中毒的金黄色葡萄球菌。结果熔解曲线分子分型体系具有良好的精密度，其批内重复性实验的标准差（s）为0．03～0．05℃，批间重复性实验的 s 为0．04～0．06℃，日间重复性实验的 s 为0．04～0．06℃。同时，熔解曲线分子分型体系能将59株金黄色葡萄球菌分为19个型别，包括11个流行克隆和8个散发克隆，Sim-pon 相关系数为0．9164。结论建立的体系快速、灵敏度高、重复性好，能应用于金黄色葡萄球菌食物中毒的快速溯源和筛查。
目的：建立金黃色葡萄毬菌鎔解麯線分子分型體繫，應用于金黃色葡萄毬菌食物中毒快速溯源。方法根據文獻閤成金黃色葡萄毬菌 VNTR 位點的相應引物，用于檢測已知 PFGE 型彆的金黃色葡萄毬菌，從中篩選齣4箇分辨力較高的位點。在此基礎上建立鎔解麯線分子分型體繫，檢測59株分離自食物中毒的金黃色葡萄毬菌。結果鎔解麯線分子分型體繫具有良好的精密度，其批內重複性實驗的標準差（s）為0．03～0．05℃，批間重複性實驗的 s 為0．04～0．06℃，日間重複性實驗的 s 為0．04～0．06℃。同時，鎔解麯線分子分型體繫能將59株金黃色葡萄毬菌分為19箇型彆，包括11箇流行剋隆和8箇散髮剋隆，Sim-pon 相關繫數為0．9164。結論建立的體繫快速、靈敏度高、重複性好，能應用于金黃色葡萄毬菌食物中毒的快速溯源和篩查。
목적：건립금황색포도구균용해곡선분자분형체계，응용우금황색포도구균식물중독쾌속소원。방법근거문헌합성금황색포도구균 VNTR 위점적상응인물，용우검측이지 PFGE 형별적금황색포도구균，종중사선출4개분변력교고적위점。재차기출상건립용해곡선분자분형체계，검측59주분리자식물중독적금황색포도구균。결과용해곡선분자분형체계구유량호적정밀도，기비내중복성실험적표준차（s）위0．03～0．05℃，비간중복성실험적 s 위0．04～0．06℃，일간중복성실험적 s 위0．04～0．06℃。동시，용해곡선분자분형체계능장59주금황색포도구균분위19개형별，포괄11개류행극륭화8개산발극륭，Sim-pon 상관계수위0．9164。결론건립적체계쾌속、령민도고、중복성호，능응용우금황색포도구균식물중독적쾌속소원화사사。
Objective To establish a molecular typing system of Staphylococcus aureus by using resolution melting for food-poi-soning fast tracing.Methods Primers were designed and synthesized according to the literature of VNTR in Staphylococcus au-reus ,and were used to perform molecular typing on the strains which had detected by PFGE,then 4 types of VNTRs were with higher discriminatory power were selected.On this basis,we established a molecular typing system for the detection of 59 Staphy-lococcus aureus isolated from food poisoning.Results The molecular typing system has good precision for detection.The standard deviation(s)of within-batch repetitive experiments were 0.03 -0.05 ℃,between-batch repetitive experiments were 0.04 -0.06℃,between-day repetitive experiments were 0.04-0.06 ℃.At the same time,the 59 strains of Staphylococcus aureus were divided into 19 types which were 11 epidemic clones and 8 sporadic clones.The correlation coefficient of Simpson was 0.916 4.Conclusion The molecular typing system for Staphylococcus aureus based on resolution melting was simple,fast and repeatable.It can be ap-plied to fast tracing and screen of Staphylococcus aureus in food poisoning.
