CAJ | 학술논문

目的：探讨聚合酶链式反应（PCR）技术在分析血流感染细菌菌谱中的应用，为血液病患儿细菌性血流感染的流行病学调查提供新的思路。方法从西安市儿童医院血液内科收集80例疑似血流感染患儿的外周静脉血标本进行血培养，同时扩增并测序细菌16 S rDNA，对血培养和 PCR 结果进行比较。结果80例血液标本中7例细菌培养阳性，阳性率为8．8％；PCR 检测出16例阳性，阳性率为20．0％，二者差异有统计学意义（χ2＝5．82，P ＜0．05）；在16例 PCR 阳性标本中，革兰阳性菌占68．7％，其中表皮葡萄球菌最多[占31．3％（5/16）]，革兰阴性菌占31．3％，以非发酵菌为主[占18．7％（3/16）]。结论16 S rDNA-PCR结合测序的方法可以很好地鉴定血流感染病原菌，可作为一种新的流行病学方法在临床应用；血液病儿童血流感染病原菌以革兰阳性球菌为主，尤以表皮葡萄球菌最常见。
목적：탐토취합매련식반응（PCR）기술재분석혈류감염세균균보중적응용，위혈액병환인세균성혈류감염적류행병학조사제공신적사로。방법종서안시인동의원혈액내과수집80례의사혈류감염환인적외주정맥혈표본진행혈배양，동시확증병측서세균16 S rDNA，대혈배양화 PCR 결과진행비교。결과80례혈액표본중7례세균배양양성，양성솔위8．8％；PCR 검측출16례양성，양성솔위20．0％，이자차이유통계학의의（χ2＝5．82，P ＜0．05）；재16례 PCR 양성표본중，혁란양성균점68．7％，기중표피포도구균최다[점31．3％（5/16）]，혁란음성균점31．3％，이비발효균위주[점18．7％（3/16）]。결론16 S rDNA-PCR결합측서적방법가이흔호지감정혈류감염병원균，가작위일충신적류행병학방법재림상응용；혈액병인동혈류감염병원균이혁란양성구균위주，우이표피포도구균최상견。
Objective To explore the value of PCR for analyzing the pathogenic spectrum of bloodstream infection(BSI)in chil-dren with blood disease,and to provide a new thought for epidemiological survey of BSI.Methods A total of 80 children with blood disease in hematology department with suspected bacterial infections were recruited in the study,whose blood samples were collect-ed and detected by using blood cultivation(BC)and 16 S rDNA-PCR.The results obtained by the two methods were compared.Re-sults Among the 80 children,the positive rate of BC and PCR were 8.8% and 20.0%,respectively.There was significant differ-ence between the two methods(χ2 =5.82,P <0.05).Gram-positive bacteria and Gram-negative bacteria accounted for 68.7% and 31.3% respectively in 16 bacteria.The predominant pathogen was Staphylococcus epidermidis (31.3%).Conclusion Compared with BC,16 S rDNA-PCR and sequencing provide a new way for analyzing the pathogenic spectrum of BSI,which might be effective for epidemiological investigation.The pathogens of BSI are mainly Gram-positive bacteria in children of hematology department,and Staphylococcus epidermidis is the most common pathogen.