国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
17期
2275-2276
,共2页
海洛因成瘾%海马%NMDA受体%大鼠
海洛因成癮%海馬%NMDA受體%大鼠
해락인성은%해마%NMDA수체%대서
heroin addiction%hippocampus%NMDA receptor%rats
目的：观察海洛因依赖大鼠海马CA3区NMDA受体NR1、NR2A亚基的表达。方法将40只SD大鼠随机分成海洛因依赖组、海洛因＋MK801组、海洛因戒断组及对照组，用逆转录聚合酶链反应（RT-PCR）技术半定量检测海马NMDA 受体NR1、NR2A 亚基mRNA 的表达。结果与对照组比较，海洛因依赖组、海洛因戒断组和海洛因＋MK801组的NR1表达明显升高（P＜0．05），且海洛因依赖组NR1的表达明显高于海洛因戒断组和海洛因＋MK801组，海洛因戒断组与海洛因＋MK801组的NR1表达差异无统计学意义（P＞0．05）。与对照组比较，海洛因＋MK801组NR2A的表达降低（P＜0．05），海洛因戒断组NR2A表达升高（P＜0．05），依赖组NR2A的表达差异无统计学意义（P＞0．05）。结论海洛因依赖可使大鼠海马CA3区NR1的表达明显上调。海洛因戒断的大鼠海马CA3区NR2A表达上调，使用 MK801干预的大鼠海马CA3区NR2A表达下调。
目的：觀察海洛因依賴大鼠海馬CA3區NMDA受體NR1、NR2A亞基的錶達。方法將40隻SD大鼠隨機分成海洛因依賴組、海洛因＋MK801組、海洛因戒斷組及對照組，用逆轉錄聚閤酶鏈反應（RT-PCR）技術半定量檢測海馬NMDA 受體NR1、NR2A 亞基mRNA 的錶達。結果與對照組比較，海洛因依賴組、海洛因戒斷組和海洛因＋MK801組的NR1錶達明顯升高（P＜0．05），且海洛因依賴組NR1的錶達明顯高于海洛因戒斷組和海洛因＋MK801組，海洛因戒斷組與海洛因＋MK801組的NR1錶達差異無統計學意義（P＞0．05）。與對照組比較，海洛因＋MK801組NR2A的錶達降低（P＜0．05），海洛因戒斷組NR2A錶達升高（P＜0．05），依賴組NR2A的錶達差異無統計學意義（P＞0．05）。結論海洛因依賴可使大鼠海馬CA3區NR1的錶達明顯上調。海洛因戒斷的大鼠海馬CA3區NR2A錶達上調，使用 MK801榦預的大鼠海馬CA3區NR2A錶達下調。
목적：관찰해락인의뢰대서해마CA3구NMDA수체NR1、NR2A아기적표체。방법장40지SD대서수궤분성해락인의뢰조、해락인＋MK801조、해락인계단조급대조조，용역전록취합매련반응（RT-PCR）기술반정량검측해마NMDA 수체NR1、NR2A 아기mRNA 적표체。결과여대조조비교，해락인의뢰조、해락인계단조화해락인＋MK801조적NR1표체명현승고（P＜0．05），차해락인의뢰조NR1적표체명현고우해락인계단조화해락인＋MK801조，해락인계단조여해락인＋MK801조적NR1표체차이무통계학의의（P＞0．05）。여대조조비교，해락인＋MK801조NR2A적표체강저（P＜0．05），해락인계단조NR2A표체승고（P＜0．05），의뢰조NR2A적표체차이무통계학의의（P＞0．05）。결론해락인의뢰가사대서해마CA3구NR1적표체명현상조。해락인계단적대서해마CA3구NR2A표체상조，사용 MK801간예적대서해마CA3구NR2A표체하조。
Objective To observe the effects of expression of NMDA receptor NR1 ,NR2A subunit in heroin dependent rats of hippocampus CA3 area.Methods 40 SD rats were randomly divided into four groups:Heroin Addiction Group,Heroin+MK801 Group,Heroin Abstinence Group and Control Group.The expression of NMDA receptor NR1 subunit mRNA were semi-quantita-tively determined by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with Control Group,the NR1 expression in the other three groups significantly increased(P<0.05).NR1 expression in Heroin Addiction Group was higher than Heroin+MK801 Group and Heroin Abstinence Group(P<0.05).There was no statistically significant difference between Heroin+MK801 Group and Heroin Abstinence Group(P>0.05).Compared with Control Group,Heroin+MK801 Group′s NR2A ex-pression reduced(P<0.05),Heroin Abstinence Group′s NR2A expression increased(P<0.05),no significantly difference was ob-served in Heroin Addiction Group(P>0.05).Conclusion Heroin addiction causes NR1 expression increase in hippocampus CA3 area of rats.The CA3 area NR2A expression ascended in Heroin Abstinence Group but reduced in the Heroin rats administrated with MK801 .
