CAJ | 학술논문

以负载1,4,7,10-四氮杂环十二烷(cyclen)的氯甲基壳聚糖树脂(merrifield chitosan resin-sup-ported cyclen,MCRC)为载体,配合 Ce(Ⅲ),得到以Ce(Ⅲ)为催化中心的改性氯甲基壳聚糖 Ce(Ⅲ)树脂(MCRC-Ce(Ⅲ)).通过茚三酮比色法、SDS-PAGE 电泳法及氨基酸自动分析仪等技术,检测 MCRC-Ce(Ⅲ)对牛血清蛋白(BSA)的水解能力.结果表明,MCRC-Ce(Ⅲ)在生理 pH 值,60℃条件下,可将 BSA 水解为短肽和氨基酸,24 h 后水解率可达88％,水解速率表观常数 K obs 为7．05×10-2 mol??L-1??h-1,重复使用6次后,仍能保持80％以上的水解能力,具有广阔的应用前景.
이부재1,4,7,10-사담잡배십이완(cyclen)적록갑기각취당수지(merrifield chitosan resin-sup-ported cyclen,MCRC)위재체,배합 Ce(Ⅲ),득도이Ce(Ⅲ)위최화중심적개성록갑기각취당 Ce(Ⅲ)수지(MCRC-Ce(Ⅲ)).통과인삼동비색법、SDS-PAGE 전영법급안기산자동분석의등기술,검측 MCRC-Ce(Ⅲ)대우혈청단백(BSA)적수해능력.결과표명,MCRC-Ce(Ⅲ)재생리 pH 치,60℃조건하,가장 BSA 수해위단태화안기산,24 h 후수해솔가체88％,수해속솔표관상수 K obs 위7．05×10-2 mol??L-1??h-1,중복사용6차후,잉능보지80％이상적수해능력,구유엄활적응용전경.
Merrifield chitosan resin-supported cyclen (MCRC)was prepared by attaching 1,4,7,10-tetraazacy-clododecane (cyclen)to Merrifield chitosan resin (MCR).Then coordination with Ce(Ⅲ)as catalytic center to be MCRC-Ce(Ⅲ).Ninhydrin reaction,SDS-PAGE,as well as automatic amino-acid analyzor were drawn to in-vestigate the hydrolyzing ability of MCRC-Ce(Ⅲ)toward BSA.The results indicated that after 24 hours,BSA can be hydrolyzed up to 88% at physiological pH,60℃,where protein was hydrolyzed into smaller peptides and amino acids,and the pseudo-first-order kinetic constant (K obs )was 7.05×10 -2 mol??L-1 ??h-1 .MCRC-Ce(Ⅲ) has a strong binding affinity with metal ions,which could be reused for 6 times while the catalytic ability main-tains 80% of the original,indicating that MCRC-Ce(Ⅲ)has a broad application prospect.