中华老年多器官疾病杂志
中華老年多器官疾病雜誌
중화노년다기관질병잡지
CHINESE JOURNAL OF MULTIPLE ORGAN DISEASES IN THE ELDERLY
2014年
8期
616-620
,共5页
李晓青%种莉%刘鹏%刘玥%唐鹏%陈丽%张欣%侯辰%李锐%郭民侠
李曉青%種莉%劉鵬%劉玥%唐鵬%陳麗%張訢%侯辰%李銳%郭民俠
리효청%충리%류붕%류모%당붕%진려%장흔%후신%리예%곽민협
肝性脑病%氨%细胞培养%超微结构
肝性腦病%氨%細胞培養%超微結構
간성뇌병%안%세포배양%초미결구
hepatic encephalopathy%ammonia%cell culture%ultrastructure
目的：通过观察肝性脑病大鼠脑部分区域及氨对体外培养大鼠神经元超微结构的改变，讨论其病理发生机制。方法选用健康雄性SD大鼠12只，随机分为肝性脑病模型组和正常对照组两组，每组6只。用电子透射显微镜观察硫代乙酰胺诱导的肝性脑病大鼠和体外氨中毒大鼠皮质神经元的超微结构。结果肝性脑病大鼠神经元细胞数量减少；神经元线粒体肿胀，尼氏体数量明显减少；可见凋亡各期表现。神经胶质细胞细胞器减少，黑质的超微结构改变程度较基底核略重。体外培养氨中毒神经元变化：神经元细胞数量明显减少；细胞明显水肿，线粒体明显肿胀，尼氏体显著减少；可见不同时期的凋亡表现。结论肝性脑病大鼠脑超微结构改变明显，其主要机制可能与氨中毒引起的神经元凋亡有关。
目的：通過觀察肝性腦病大鼠腦部分區域及氨對體外培養大鼠神經元超微結構的改變，討論其病理髮生機製。方法選用健康雄性SD大鼠12隻，隨機分為肝性腦病模型組和正常對照組兩組，每組6隻。用電子透射顯微鏡觀察硫代乙酰胺誘導的肝性腦病大鼠和體外氨中毒大鼠皮質神經元的超微結構。結果肝性腦病大鼠神經元細胞數量減少；神經元線粒體腫脹，尼氏體數量明顯減少；可見凋亡各期錶現。神經膠質細胞細胞器減少，黑質的超微結構改變程度較基底覈略重。體外培養氨中毒神經元變化：神經元細胞數量明顯減少；細胞明顯水腫，線粒體明顯腫脹，尼氏體顯著減少；可見不同時期的凋亡錶現。結論肝性腦病大鼠腦超微結構改變明顯，其主要機製可能與氨中毒引起的神經元凋亡有關。
목적：통과관찰간성뇌병대서뇌부분구역급안대체외배양대서신경원초미결구적개변，토론기병리발생궤제。방법선용건강웅성SD대서12지，수궤분위간성뇌병모형조화정상대조조량조，매조6지。용전자투사현미경관찰류대을선알유도적간성뇌병대서화체외안중독대서피질신경원적초미결구。결과간성뇌병대서신경원세포수량감소；신경원선립체종창，니씨체수량명현감소；가견조망각기표현。신경효질세포세포기감소，흑질적초미결구개변정도교기저핵략중。체외배양안중독신경원변화：신경원세포수량명현감소；세포명현수종，선립체명현종창，니씨체현저감소；가견불동시기적조망표현。결론간성뇌병대서뇌초미결구개변명현，기주요궤제가능여안중독인기적신경원조망유관。
ObjectiveTo observe the ultrastructurechangeof the brain tissues in the rats with hepatic encephalopathy and determine thein vitroeffect of ammonia on the ultrastructure of rat neurons in order to investigate the pathological mechanism of these changes.MethodsTwelve healthy male SD rats were randomly divided into two groups: hepatic encephalopathy group and normal control group (with six in each group). Transmission electron microscopy was employed to observe the ultrastructureof the brain tissuesin rats with hepatic encephalopathyinducedby thioacetamide and ofin vitro cultured rat neuronsinducedby ammonia. ResultsIn rats with hepatic encephalopathy, theneuronswere reducedinnumber,withswollen mitochondria, significantly decreasedNissl body quantity,and arrestedin each stage ofapoptosis.While, the cell organelles in glialwerealso decreased, and the ultrastructure changes were more serious in the nigra thaninthebasal nuclei.Forin vitroinjured rat neuronsinducedby ammonia, theneurons were decreased in number,withedema, swollen mitochondria,andsignificantly reducedNissl body quantity,and displayed apoptosisat each stage.Conclusion Obvious changes are seen in theultrastructure of nervouscells in rats with hepatic encephalopathyandin vitroinjured neurons, which might be associated withneuron apoptosis caused by ammonia.
